Axon-target interactions maintain synaptic gene expression in retinae transplanted to intracranial regions of the rat

F. Hoover, Mark Hankin, J. D. Radel, J. S. Reese, D. Goldman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In the present study we examined the effects of optic axon-CNS target interactions on gene expression in the rat retina. These studies took advantage of a transplantation paradigm that allowed us to assay gene expression in retinae transplanted to different intracranial locations in the neonatal rat that either promoted (dorsal midbrain) or precluded (cerebral cortex) the formation of retino-collicular connections. Using in situ hybridization experiments, we observed that transplantation to the dorsal midbrain resulted in a relatively normal pattern of nicotinic acetylcholine receptor (nAChR) β-3 subunit and glutamate receptor 3 (GluR3) gene expression. In contrast, retinae transplanted to the cerebral cortex (which did not result in normal retino-collicular interactions) showed a dramatic reduction in nAChR β-3 subunit and GluR3 gene expression. These results agree with those obtained in the adult goldfish retina, where it has been demonstrated that an optic nerve-optic tectum interaction is responsible for the re-induction nAChR and NMDA receptor gene expression during optic nerve regeneration. Taken together, these results support the hypothesis that proper axon-target interactions are required for maintenance of nAChR and glutamate receptor gene expression in the mature vertebrate retina.

Original languageEnglish (US)
Pages (from-to)123-132
Number of pages10
JournalMolecular Brain Research
Volume51
Issue number1-2
DOIs
StatePublished - Nov 1 1997
Externally publishedYes

Fingerprint

Axons
Retina
Nicotinic Receptors
Gene Expression
Optic Nerve
Mesencephalon
Cerebral Cortex
Transplantation
Nerve Regeneration
Goldfish
Superior Colliculi
Glutamate Receptors
N-Methyl-D-Aspartate Receptors
In Situ Hybridization
Vertebrates
Maintenance
AMPA 3 glutamate receptor ionotropic

Keywords

  • Gene expression
  • Glutamate receptor
  • Hybridization, in situ
  • Nicotinic acetylcholine receptor
  • Retinal ganglion cell

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Axon-target interactions maintain synaptic gene expression in retinae transplanted to intracranial regions of the rat. / Hoover, F.; Hankin, Mark; Radel, J. D.; Reese, J. S.; Goldman, D.

In: Molecular Brain Research, Vol. 51, No. 1-2, 01.11.1997, p. 123-132.

Research output: Contribution to journalArticle

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