Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production

Makiko Umezu-Goto, Yasuhiro Kishi, Akitsu Taira, Kotaro Hama, Naoshi Dohmae, Koji Takio, Takao Yamori, Gordon Mills, Keizo Inoue, Junken Aoki, Hiroyuki Arai

Research output: Contribution to journalArticle

684 Citations (Scopus)

Abstract

Autotaxin (ATX) is a tumor cell motility-stimulating factor, originally isolated from melanoma cell supernatants. ATX had been proposed to mediate its effects through 5'-nucleotide pyrophosphatase and phosphodiesterase activities. However, the ATX substrate mediating the increase in cellular motility remains to be identified. Here, we demonstrated that lysophospholipase D (lysoPLD) purified from fetal bovine serum, which catalyzes the production of the bioactive phospholipid mediator, lysophosphatidic acid (LPA), from lysophosphatidylcholine (LPC), is identical to ATX. The Km value of ATX for LPC was 25-fold lower than that for the synthetic nucleoside substrate, ρ-nitrophenyl-tri-monophosphate. LPA mediates multiple biological functions including cytoskeletal reorganization, chemotaxis, and cell growth through activation of specific G protein-coupled receptors. Recombinant ATX, particularly in the presence of LPC, dramatically increased chemotaxis and proliferation of multiple different cell lines. Moreover, we demonstrate that several cancer cell lines release significant amounts of LPC, a substrate for ATX, into the culture medium. The demonstration that ATX and lysoPLD are identical suggests that autocrine or paracrine production of LPA contributes to tumor cell motility, survival, and proliferation. It also provides potential novel targets for therapy of pathophysiological states including cancer.

Original languageEnglish (US)
Pages (from-to)227-233
Number of pages7
JournalJournal of Cell Biology
Volume158
Issue number2
DOIs
StatePublished - Jul 22 2002
Externally publishedYes

Fingerprint

Lysophosphatidylcholines
Cell Movement
Chemotaxis
Growth
nucleotide pyrophosphatase
Neoplasms
Cell Line
Phosphoric Diester Hydrolases
G-Protein-Coupled Receptors
Nucleosides
Culture Media
Melanoma
Phospholipids
Cell Survival
Cell Proliferation
lysophosphatidic acid
alkylglycerophosphoethanolamine phosphodiesterase
Serum
Therapeutics

Keywords

  • Cell proliferation
  • Chemotaxis
  • EDG receptor
  • Lysophosphatidylcholine
  • LysoPLD

ASJC Scopus subject areas

  • Cell Biology

Cite this

Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production. / Umezu-Goto, Makiko; Kishi, Yasuhiro; Taira, Akitsu; Hama, Kotaro; Dohmae, Naoshi; Takio, Koji; Yamori, Takao; Mills, Gordon; Inoue, Keizo; Aoki, Junken; Arai, Hiroyuki.

In: Journal of Cell Biology, Vol. 158, No. 2, 22.07.2002, p. 227-233.

Research output: Contribution to journalArticle

Umezu-Goto, M, Kishi, Y, Taira, A, Hama, K, Dohmae, N, Takio, K, Yamori, T, Mills, G, Inoue, K, Aoki, J & Arai, H 2002, 'Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production', Journal of Cell Biology, vol. 158, no. 2, pp. 227-233. https://doi.org/10.1083/jcb.200204026
Umezu-Goto, Makiko ; Kishi, Yasuhiro ; Taira, Akitsu ; Hama, Kotaro ; Dohmae, Naoshi ; Takio, Koji ; Yamori, Takao ; Mills, Gordon ; Inoue, Keizo ; Aoki, Junken ; Arai, Hiroyuki. / Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production. In: Journal of Cell Biology. 2002 ; Vol. 158, No. 2. pp. 227-233.
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