Autoradiolysis of iodinated monoclonal antibody preparations

R. Kishore, J. F. Eary, Kenneth Krohn, W. B. Nelp, T. W. Menard, P. L. Beaumier, K. E. Hellström, I. Hellström

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We investigated the effects of ionizing radiation on the immunointegrity of antibody fragments (Fab) because large amounts of high specific activity 131I may damage the proteins. We found that 1000 Gy of external 137Cs γ radiation was sufficient to destroy 80-90% of the immunointegrity of the initial preparation. This effect was also produced by internally added [131I]NaI in a quantity sufficient to provide the same radiation absorbed dose. Since radioiodinated monoclonal antibodies labeled to high specific activity are being evaluated for radioimmunotherapy, the above observation is significant since high levels of internal radiation occur with therapeutic doses of 131I-labeled antibody. Human serum albumin in low concentration (2%) added to the iodinated antibody solutions was successful in preventing loss of immunoreactivity and can be used to protect and stabilize therapeutic quantities of radiolabeled monoclonal antibody preparations.

Original languageEnglish (US)
Pages (from-to)457-459
Number of pages3
JournalNuclear Medicine and Biology
Volume13
Issue number4
StatePublished - 1986
Externally publishedYes

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Monoclonal Antibodies
Radiation
Radioimmunotherapy
Immunoglobulin Fragments
Antibodies
Ionizing Radiation
Serum Albumin
Observation
Therapeutics
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

Kishore, R., Eary, J. F., Krohn, K., Nelp, W. B., Menard, T. W., Beaumier, P. L., ... Hellström, I. (1986). Autoradiolysis of iodinated monoclonal antibody preparations. Nuclear Medicine and Biology, 13(4), 457-459.

Autoradiolysis of iodinated monoclonal antibody preparations. / Kishore, R.; Eary, J. F.; Krohn, Kenneth; Nelp, W. B.; Menard, T. W.; Beaumier, P. L.; Hellström, K. E.; Hellström, I.

In: Nuclear Medicine and Biology, Vol. 13, No. 4, 1986, p. 457-459.

Research output: Contribution to journalArticle

Kishore, R, Eary, JF, Krohn, K, Nelp, WB, Menard, TW, Beaumier, PL, Hellström, KE & Hellström, I 1986, 'Autoradiolysis of iodinated monoclonal antibody preparations', Nuclear Medicine and Biology, vol. 13, no. 4, pp. 457-459.
Kishore R, Eary JF, Krohn K, Nelp WB, Menard TW, Beaumier PL et al. Autoradiolysis of iodinated monoclonal antibody preparations. Nuclear Medicine and Biology. 1986;13(4):457-459.
Kishore, R. ; Eary, J. F. ; Krohn, Kenneth ; Nelp, W. B. ; Menard, T. W. ; Beaumier, P. L. ; Hellström, K. E. ; Hellström, I. / Autoradiolysis of iodinated monoclonal antibody preparations. In: Nuclear Medicine and Biology. 1986 ; Vol. 13, No. 4. pp. 457-459.
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