Autophagic Killing Effects against Mycobacterium tuberculosis by Alveolar Macrophages from Young and Aged Rhesus Macaques

Sophia A. Pacheco, Katelyn M. Powers, Flora Engelmann, Ilhem Messaoudi, Georgiana Purdy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Non-human primates, notably rhesus macaques (Macaca mulatta, RM), provide a robust experimental model to investigate the immune response to and effective control of Mycobacterium tuberculosis infections. Changes in the function of immune cells and immunosenescence may contribute to the increased susceptibility of the elderly to tuberculosis. The goal of this study was to examine the impact of age on M. tuberculosis host-pathogen interactions following infection of primary alveolar macrophages derived from young and aged rhesus macaques. Of specific interest to us was whether the mycobactericidal capacity of autophagic macrophages was reduced in older animals since decreased autophagosome formation and autophagolysosomal fusion has been observed in other cells types of aged animals. Our data demonstrate that alveolar macrophages from old RM are as competent as those from young animals for autophagic clearance of M. tuberculosis infection and controlling mycobacterial replication. While our data do not reveal significant differences between alveolar macrophage responses to M. tuberculosis by young and old animals, these studies are the first to functionally characterize autophagic clearance of M. tuberculosis by alveolar macrophages from RM.

Original languageEnglish (US)
Article numbere66985
JournalPLoS One
Volume8
Issue number6
DOIs
StatePublished - Jun 18 2013

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Alveolar Macrophages
Mycobacterium tuberculosis
Macaca mulatta
Animals
macrophages
Mycobacterium Infections
young animals
Macrophages
infection
Pathogens
Host-Pathogen Interactions
host-pathogen relationships
Fusion reactions
tuberculosis
Primates
Tuberculosis
Theoretical Models
immune response
cells
Infection

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Autophagic Killing Effects against Mycobacterium tuberculosis by Alveolar Macrophages from Young and Aged Rhesus Macaques. / Pacheco, Sophia A.; Powers, Katelyn M.; Engelmann, Flora; Messaoudi, Ilhem; Purdy, Georgiana.

In: PLoS One, Vol. 8, No. 6, e66985, 18.06.2013.

Research output: Contribution to journalArticle

Pacheco, Sophia A. ; Powers, Katelyn M. ; Engelmann, Flora ; Messaoudi, Ilhem ; Purdy, Georgiana. / Autophagic Killing Effects against Mycobacterium tuberculosis by Alveolar Macrophages from Young and Aged Rhesus Macaques. In: PLoS One. 2013 ; Vol. 8, No. 6.
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