AUTOMATION OF CYTOGENETICS. ASILOMAR WORKSHOP, PACIFIC GROVE, CALIFORNIA: current status and potential development

M. A. Van Dilla, A. V. Carrano, Joe Gray

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Suspensions of metaphase chromosomes from chinese hamster, Indian muntjac and human tissue culture cells were stained for DNA with the fluorochrome, ethidium bromide, and analyzed by flow cytometry to produce a DNA distribution or flow karyotype unique for each species. The flow karyotype is a representation of chromosomal DNA content and relative chromosome frequency. The time for the measurement and data reduction is about ten minutes per 106 chromosomes. Aberrant chromosomes have been detected by flow karyotype changes. Improvements of flow karyotyping and the feasibility of this technique for monitoring human populations for cytogenetic alterations are discussed.

Original languageEnglish (US)
Title of host publicationLAWRENCE LIVERMORE LAB., U.CALIF.
Pages145-164
Number of pages20
StatePublished - 1976
Externally publishedYes

Fingerprint

Automation
Cytogenetics
Chromosomes
Karyotype
Education
DNA
Muntjacs
Karyotyping
Ethidium
Metaphase
Cricetulus
Fluorescent Dyes
Suspensions
Flow Cytometry
Cell Culture Techniques
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Van Dilla, M. A., Carrano, A. V., & Gray, J. (1976). AUTOMATION OF CYTOGENETICS. ASILOMAR WORKSHOP, PACIFIC GROVE, CALIFORNIA: current status and potential development. In LAWRENCE LIVERMORE LAB., U.CALIF. (pp. 145-164)

AUTOMATION OF CYTOGENETICS. ASILOMAR WORKSHOP, PACIFIC GROVE, CALIFORNIA : current status and potential development. / Van Dilla, M. A.; Carrano, A. V.; Gray, Joe.

LAWRENCE LIVERMORE LAB., U.CALIF.. 1976. p. 145-164.

Research output: Chapter in Book/Report/Conference proceedingChapter

Van Dilla, MA, Carrano, AV & Gray, J 1976, AUTOMATION OF CYTOGENETICS. ASILOMAR WORKSHOP, PACIFIC GROVE, CALIFORNIA: current status and potential development. in LAWRENCE LIVERMORE LAB., U.CALIF.. pp. 145-164.
Van Dilla MA, Carrano AV, Gray J. AUTOMATION OF CYTOGENETICS. ASILOMAR WORKSHOP, PACIFIC GROVE, CALIFORNIA: current status and potential development. In LAWRENCE LIVERMORE LAB., U.CALIF.. 1976. p. 145-164
Van Dilla, M. A. ; Carrano, A. V. ; Gray, Joe. / AUTOMATION OF CYTOGENETICS. ASILOMAR WORKSHOP, PACIFIC GROVE, CALIFORNIA : current status and potential development. LAWRENCE LIVERMORE LAB., U.CALIF.. 1976. pp. 145-164
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