Automated counting of T cell rosettes

Arthur A. Vandenbark, R. Mark Vetto

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Human T cell rosettes were enumerated using an automated particle counter, the Bio/Physics Cytograf 6300A. An electronic oscilloscope representation of particle absorbance and scatter of a focused laser beam allows the separation and enumeration of both rosetted and non-rosetted lymphocytes. Repeated cytograf sampling of a single rosette preparation gave highly reproducible results, and sampling from replicate tubes produced the same degree of variation as microscopic analysis. T cell rosettes prepared from 27 volunteers and compared by both methods of quantitation showed a high degree of correlation. This method can objectively measure at least 100 times as many cells for their rosette-forming capability as the tedious microscopic technique.

Original languageEnglish (US)
Pages (from-to)261-270
Number of pages10
JournalJournal of Immunological Methods
Volume10
Issue number2-3
DOIs
StatePublished - Mar 1976

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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