Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis

David Seifer, M. R. Freeman, A. C. Gardiner, G. A. Hill, A. L. Schneyer, B. C. Vanderhyden

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: To determine if embryos can modulate steroid hormone production by luteinized granulosa cells. Design: Granulosa cells obtained from follicular aspirates were cultured alone or in the presence of a two- pronuclear zygote. The production of E2 and P by these cultures was evaluated by RIA. Setting: In Vitro Fertilization Unit in an academic research environment. Patients: Sixteen women undergoing IVF. Interventions: Standard IVF-ET treatment cycle using leuprolide acetate for pituitary desensitization before hMG or urofollitropin for ovarian stimulation. Main Outcome Measures: Estradiol and P concentration in culture media of luteinized granulosa cells alone or granulosa cells cocultured with a two- pronuclear embryo. Results: Both E2 and P production by luteinized granulosa cells was reduced when cultured in the presence of an embryo. Conclusions: Human embryos secrete a factor that regulates granulosa cell steroidogenesis.

Original languageEnglish (US)
Pages (from-to)425-429
Number of pages5
JournalFertility and Sterility
Volume66
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

Granulosa Cells
Zygote
Coculture Techniques
Embryonic Structures
Urofollitropin
Leuprolide
Ovulation Induction
Fertilization in Vitro
Culture Media
Estradiol
Steroids
Outcome Assessment (Health Care)
Hormones
Research

Keywords

  • autologous granulosa cell coculture
  • embryo development
  • estradiol
  • Granulosa cell steroidogenesis
  • progesterone

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Seifer, D., Freeman, M. R., Gardiner, A. C., Hill, G. A., Schneyer, A. L., & Vanderhyden, B. C. (1996). Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis. Fertility and Sterility, 66(3), 425-429.

Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis. / Seifer, David; Freeman, M. R.; Gardiner, A. C.; Hill, G. A.; Schneyer, A. L.; Vanderhyden, B. C.

In: Fertility and Sterility, Vol. 66, No. 3, 1996, p. 425-429.

Research output: Contribution to journalArticle

Seifer, D, Freeman, MR, Gardiner, AC, Hill, GA, Schneyer, AL & Vanderhyden, BC 1996, 'Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis', Fertility and Sterility, vol. 66, no. 3, pp. 425-429.
Seifer D, Freeman MR, Gardiner AC, Hill GA, Schneyer AL, Vanderhyden BC. Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis. Fertility and Sterility. 1996;66(3):425-429.
Seifer, David ; Freeman, M. R. ; Gardiner, A. C. ; Hill, G. A. ; Schneyer, A. L. ; Vanderhyden, B. C. / Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis. In: Fertility and Sterility. 1996 ; Vol. 66, No. 3. pp. 425-429.
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