Autoimmunity to a 28-30 kD cell membrane DNA binding protein

Occurrence in selected sera from patients with SLE and mixed connective tissue disease (MCTD)

Robert (Rob) Bennett, K. A. Cornell, M. J. Merritt, A. C. Bakke, P. H. Hsu, Steven Hefeneider

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Previous experiments have established the presence of a 30-kD DNA binding protein on the surface of human leukocytes. Herein we report that selected sera from patients with systemic lupus erythematosus (SLE) and MCTD are reactive with a 28-30 kD protein on immunoblots of peripheral blood mononuclear cells (PBMC) cell membrane preparations; the reactivity is abolished by prior incubation of the blot with DNA. Antibodies eluted from the 28-30 kD strip inhibited the binding of 3H.DNA to human PBMC. An immunomatrix of 28-30 kD reactive immunoglobulins was able to extract a 29-kD DNA binding protein from a PBMC cell membrane preparation. Flow cytometry experiments confirmed the cell surface IgG reactivity of sera with T lymphocytes. Additional experiments indicated that cell surface IgG binding was not due to antibodies binding to cell surface DNA, DNA anti-DNA immune complexes reacting with a DNA binding protein, anti-histone antibodies or anti-Sm antibodies. It is hypothesized that this autoimmune response could be one component of an idiotypic network involving anti-DNA antibodies.

    Original languageEnglish (US)
    Pages (from-to)374-379
    Number of pages6
    JournalClinical and Experimental Immunology
    Volume86
    Issue number3
    StatePublished - 1991

    Fingerprint

    Mixed Connective Tissue Disease
    DNA-Binding Proteins
    Autoimmunity
    Systemic Lupus Erythematosus
    Membrane Proteins
    Cell Membrane
    DNA
    Blood Cells
    Serum
    Anti-Idiotypic Antibodies
    Immunoglobulin G
    Antibodies
    Antinuclear Antibodies
    Antigen-Antibody Complex
    Histones
    Immunoglobulins
    Flow Cytometry
    Leukocytes
    T-Lymphocytes
    Proteins

    Keywords

    • Antibodies
    • Cell surface
    • DNA binding protein
    • MCTD
    • SLE

    ASJC Scopus subject areas

    • Immunology

    Cite this

    Autoimmunity to a 28-30 kD cell membrane DNA binding protein : Occurrence in selected sera from patients with SLE and mixed connective tissue disease (MCTD). / Bennett, Robert (Rob); Cornell, K. A.; Merritt, M. J.; Bakke, A. C.; Hsu, P. H.; Hefeneider, Steven.

    In: Clinical and Experimental Immunology, Vol. 86, No. 3, 1991, p. 374-379.

    Research output: Contribution to journalArticle

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