Augmentation of coronary conductance in adult sheep made anaemic during fetal life

Lowell Davis, Jean-Baptiste Roullet, Kent Thornburg, M. Shokry, Alan (Roger) Hohimer, George Giraud

Research output: Contribution to journalReview article

33 Citations (Scopus)

Abstract

Maximal coronary conductance with adenosine in anaemic fetal sheep is twice that of non-anaemic fetuses. To investigate whether this increase in conductance persists into adulthood we studied twin sheep as fetuses and again as adults. Nine anaemic fetuses (118 days gestation) underwent isovolaemic haemorrhage for 18.0 ± 4.6 days (means ± S.D.) during which time the haematocrit was reduced from 39.9 ± 5.2% to 16.3 ± 3.4% and oxygen content from 8.6 ± 1.3 to 2.3 ± 0.2 ml dl-1. At 138 days the anaemic fetuses were transfused; at delivery the haematocrit was 29.3 ± 6.8% compared to nine control fetuses in which the haematocrit was 38.5 ± 4.3%. The weight at delivery was 3.5 ± 0.36 kg in the anaemic fetuses vs. 4.2 ± 0.83 kg in controls. Twenty-eight weeks later, we placed an occluder on the descending thoracic aorta and inferior vena cava, a flow probe around the proximal left circumflex coronary artery, and catheters in the left atrial appendage, jugular and carotid vessels. Maximal coronary conductance was determined in the adults by recording coronary blood flow as driving pressure was altered by inflating the occluders while adenosine was infused into the left atrium. Right atrial, left atrial, systolic and mean arterial pressures, systemic vascular resistance and haematocrit were not different between 'in utero anaemic' and control adults. The adults that were anaemic in utero weighed less than the controls 39.4 ± 4.6 kg vs. 45.0 ± 5.6 kg. Maximal conductance was greater in the adults that were anaemic in utero: 11.2 ± 4.0 ml min-1 (100 g)-1 mmHg-1 as compared to 6.1 ± 1.8 ml min-1 (100 g)-1 mmHg-1 in the controls. Vascular reactivity of the mesenteric: arteries was not different. These data suggest that coronary conductance can be modified in utero by anaemia (high flow and hypoxaemia) and that the remodelled coronary tree persists to adulthood.

Original languageEnglish (US)
Pages (from-to)53-59
Number of pages7
JournalJournal of Physiology
Volume547
Issue number1
DOIs
StatePublished - Feb 15 2003

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Sheep
Fetus
Hematocrit
Thoracic Aorta
Adenosine
Atrial Appendage
Mesenteric Arteries
Inferior Vena Cava
Heart Atria
Vascular Resistance
Blood Vessels
Anemia
Coronary Vessels
Arterial Pressure
Neck
Catheters
Hemorrhage
Oxygen
Pressure
Weights and Measures

ASJC Scopus subject areas

  • Physiology

Cite this

Augmentation of coronary conductance in adult sheep made anaemic during fetal life. / Davis, Lowell; Roullet, Jean-Baptiste; Thornburg, Kent; Shokry, M.; Hohimer, Alan (Roger); Giraud, George.

In: Journal of Physiology, Vol. 547, No. 1, 15.02.2003, p. 53-59.

Research output: Contribution to journalReview article

Davis, Lowell ; Roullet, Jean-Baptiste ; Thornburg, Kent ; Shokry, M. ; Hohimer, Alan (Roger) ; Giraud, George. / Augmentation of coronary conductance in adult sheep made anaemic during fetal life. In: Journal of Physiology. 2003 ; Vol. 547, No. 1. pp. 53-59.
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abstract = "Maximal coronary conductance with adenosine in anaemic fetal sheep is twice that of non-anaemic fetuses. To investigate whether this increase in conductance persists into adulthood we studied twin sheep as fetuses and again as adults. Nine anaemic fetuses (118 days gestation) underwent isovolaemic haemorrhage for 18.0 ± 4.6 days (means ± S.D.) during which time the haematocrit was reduced from 39.9 ± 5.2{\%} to 16.3 ± 3.4{\%} and oxygen content from 8.6 ± 1.3 to 2.3 ± 0.2 ml dl-1. At 138 days the anaemic fetuses were transfused; at delivery the haematocrit was 29.3 ± 6.8{\%} compared to nine control fetuses in which the haematocrit was 38.5 ± 4.3{\%}. The weight at delivery was 3.5 ± 0.36 kg in the anaemic fetuses vs. 4.2 ± 0.83 kg in controls. Twenty-eight weeks later, we placed an occluder on the descending thoracic aorta and inferior vena cava, a flow probe around the proximal left circumflex coronary artery, and catheters in the left atrial appendage, jugular and carotid vessels. Maximal coronary conductance was determined in the adults by recording coronary blood flow as driving pressure was altered by inflating the occluders while adenosine was infused into the left atrium. Right atrial, left atrial, systolic and mean arterial pressures, systemic vascular resistance and haematocrit were not different between 'in utero anaemic' and control adults. The adults that were anaemic in utero weighed less than the controls 39.4 ± 4.6 kg vs. 45.0 ± 5.6 kg. Maximal conductance was greater in the adults that were anaemic in utero: 11.2 ± 4.0 ml min-1 (100 g)-1 mmHg-1 as compared to 6.1 ± 1.8 ml min-1 (100 g)-1 mmHg-1 in the controls. Vascular reactivity of the mesenteric: arteries was not different. These data suggest that coronary conductance can be modified in utero by anaemia (high flow and hypoxaemia) and that the remodelled coronary tree persists to adulthood.",
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AB - Maximal coronary conductance with adenosine in anaemic fetal sheep is twice that of non-anaemic fetuses. To investigate whether this increase in conductance persists into adulthood we studied twin sheep as fetuses and again as adults. Nine anaemic fetuses (118 days gestation) underwent isovolaemic haemorrhage for 18.0 ± 4.6 days (means ± S.D.) during which time the haematocrit was reduced from 39.9 ± 5.2% to 16.3 ± 3.4% and oxygen content from 8.6 ± 1.3 to 2.3 ± 0.2 ml dl-1. At 138 days the anaemic fetuses were transfused; at delivery the haematocrit was 29.3 ± 6.8% compared to nine control fetuses in which the haematocrit was 38.5 ± 4.3%. The weight at delivery was 3.5 ± 0.36 kg in the anaemic fetuses vs. 4.2 ± 0.83 kg in controls. Twenty-eight weeks later, we placed an occluder on the descending thoracic aorta and inferior vena cava, a flow probe around the proximal left circumflex coronary artery, and catheters in the left atrial appendage, jugular and carotid vessels. Maximal coronary conductance was determined in the adults by recording coronary blood flow as driving pressure was altered by inflating the occluders while adenosine was infused into the left atrium. Right atrial, left atrial, systolic and mean arterial pressures, systemic vascular resistance and haematocrit were not different between 'in utero anaemic' and control adults. The adults that were anaemic in utero weighed less than the controls 39.4 ± 4.6 kg vs. 45.0 ± 5.6 kg. Maximal conductance was greater in the adults that were anaemic in utero: 11.2 ± 4.0 ml min-1 (100 g)-1 mmHg-1 as compared to 6.1 ± 1.8 ml min-1 (100 g)-1 mmHg-1 in the controls. Vascular reactivity of the mesenteric: arteries was not different. These data suggest that coronary conductance can be modified in utero by anaemia (high flow and hypoxaemia) and that the remodelled coronary tree persists to adulthood.

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