Attenuation of the formation of DNA-repair foci containing RAD51 in Fanconi anaemia

Martin Digweed, Susanne Rothe, Ilja Demuth, Regina Scholz, Detlev Schindler, Markus Stumm, Markus Grompe, Andreas Jordan, Karl Sperling

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

The role of the Fanconi anaemia genes in DNA repair was examined by a quantitative analysis of nuclear DNA repair foci in FA primary fibroblasts after ionising irradiation using antibodies directed against RAD51, MRE11 and BRCA1 for visualisation. IR induced foci detected with anti-RAD51, but not those detected with anti-MRE11, are reduced in fibroblasts of all eight FA complementation groups in comparison to control cells. Correction of FA-A, FA-C and FA-G cells by retroviral cDNA transfer specifically corrected the RAD51-foci response but did not affect formation of foci containing BRCA1 or MRE11. Since all FA cells, except FA-D1, lack the monoubiquitinated FANCD2-L protein, this isoform is likely to be involved in the formation of nuclear foci containing RAD51 in diploid FA cells. FA-D1 cells show the same attenuation in RAD51 foci formation, suggesting that the unknown FANCD1 protein is similarly involved in RAD51 foci formation, either independently or as a subsequent step in the FANCD2 pathway. These findings indicate that Fanconi anaemia cells have an impairment in the RAD51-dependent homologous recombination pathway for DNA repair, explaining their chromosomal instability and extreme sensitivity to DNA cross-linking agents.

Original languageEnglish (US)
Pages (from-to)1121-1126
Number of pages6
JournalCarcinogenesis
Volume23
Issue number7
StatePublished - Jan 1 2002

ASJC Scopus subject areas

  • Cancer Research

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