Attenuated-affinity biotin analogs for catch-and-release with streptavidin

L. Ying, B. Branchaud, S. Corry

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The binding between streptavin and biotin, which is one of the strongest non-covalent interactions known in nature, is used ubiquitously in molecular biology. However, when a biotinylated molecule is captured for testing purposes and cannot be easily removed from the streptavidin-biotin complex, the extreme binding strength is a disadvantage. A successfully engineered streptavin-biotin system for capture and release of sensitive biomaterials must have 1) a strong binding interaction, meaning a low equilibrium binding constant, KD; 2) a fast on-rate, ka, permitting formation of a binding complex within short incubation times; 3) a fast off-rate, kd, permitting rapid re-equilibration of the system upon introducing a competitive binder. Here we present several biotin analogs that have attenuated affinities for streptavidin. Using these compounds, we demonstrate several experimental techniques that were not feasible with prior streptavidin or biotin analogs.

Original languageEnglish (US)
Title of host publicationTechnical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
Pages151-154
Number of pages4
StatePublished - Nov 23 2011
EventNanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 - Boston, MA, United States
Duration: Jun 13 2011Jun 16 2011

Publication series

NameTechnical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
Volume3

Conference

ConferenceNanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
CountryUnited States
CityBoston, MA
Period6/13/116/16/11

Keywords

  • Affinity
  • Binding
  • Biotin
  • Kinetics
  • Streptavidin

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Ying, L., Branchaud, B., & Corry, S. (2011). Attenuated-affinity biotin analogs for catch-and-release with streptavidin. In Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 (pp. 151-154). (Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011; Vol. 3).