Atomic force microscopy study of the structure-function relationships of the biofilm-forming bacterium Streptococcus mutans

Sarah E. Cross, Jens Kreth, Lin Zhu, Fengxia Qi, Andrew E. Pelling, Wenyuan Shi, James K. Gimzewski

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Atomic force microscopy (AFM) has garnered much interest in recent years for its ability to probe the structure, function and cellular nanomechanics inherent to specific biological cells. In particular, we have used AFM to probe the important structure-function relationships of the bacterium Streptococcus mutans. S. mutans is the primary aetiological agent in human dental caries (tooth decay), and is of medical importance due to the virulence properties of these cells in biofilm initiation and formation, leading to increased tolerance to antibiotics. We have used AFM to characterize the unique surface structures of distinct mutants of S. mutans. These mutations are located in specific genes that encode surface proteins, thus using AFM we have resolved characteristic surface features for mutant strains compared to the wild type. Ultimately, our characterization of surface morphology has shown distinct differences in the local properties displayed by various S. mutans strains on the nanoscale, which is imperative for understanding the collective properties of these cells in biofilm formation.

Original languageEnglish (US)
JournalNanotechnology
Volume17
Issue number4
DOIs
StatePublished - Feb 28 2006
Externally publishedYes

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Biofilms
Atomic force microscopy
Bacteria
Nanomechanics
Antibiotics
Surface structure
Surface morphology
Membrane Proteins
Genes
Anti-Bacterial Agents
Proteins

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Atomic force microscopy study of the structure-function relationships of the biofilm-forming bacterium Streptococcus mutans. / Cross, Sarah E.; Kreth, Jens; Zhu, Lin; Qi, Fengxia; Pelling, Andrew E.; Shi, Wenyuan; Gimzewski, James K.

In: Nanotechnology, Vol. 17, No. 4, 28.02.2006.

Research output: Contribution to journalArticle

Cross, Sarah E. ; Kreth, Jens ; Zhu, Lin ; Qi, Fengxia ; Pelling, Andrew E. ; Shi, Wenyuan ; Gimzewski, James K. / Atomic force microscopy study of the structure-function relationships of the biofilm-forming bacterium Streptococcus mutans. In: Nanotechnology. 2006 ; Vol. 17, No. 4.
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