Ataxia and c-Fos expression in mice drinking ethanol in a limited access session

Amanda L. Sharpe, Natalia O. Tsivkovskaia, Andrey Ryabinin

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Background: Although previous murine studies have demonstrated ethanol self-administration resulting in blood ethanol concentrations (BECs) believed to be pharmacologically relevant, to our knowledge, no study reported to date has demonstrated intoxication via ataxia after self-administration. Thus, the goal of this study was to demonstrate ataxia and to examine changes in c-Fos expression in mice after self-administration of intoxicating doses of ethanol. Methods: Male C57BL/6J mice were trained to drink a 10% ethanol solution during daily 30-min limited access sessions. Mice were exposed to increasing concentrations of ethanol until a 10% ethanol solution was reached. BEC and ataxia, measured as foot slips off of a balance beam, were examined after the limited access self-administration session. In a separate experiment, various brain structures from mice drinking water or ethanol were examined for changes in c-Fos expression two hr after the limited access session. Results: Mice drank between 1.5 and 2 g/kg of 10% ethanol during the daily 30-min session. BECs for these mice 15 min after the limited access session ranged between 0.52 and 2.13 mg/ml. A significant increase in foot slips off a balance beam was seen immediately after ethanol consumption during the limited access session. Among mice drinking ethanol, an increase in c-Fos expression was seen in the Edinger-Westphal nucleus, and a decrease in c-Fos expression was seen in the cingulate cortex, ventral tegmental area, lateral and medial septum, CA1 region of the hippocampus, and basolateral amygdala. Conclusions: After this procedure in mice, BECs are achieved that are in a range considered pharmacologically relevant and intoxicating. Significant ataxia was observed after ethanol self-administration. Brain regions showing changes in c-Fos expression after voluntary intoxication were similar to those previously reported, suggesting that these brain regions are involved in regulating behavioral effects of alcohol intoxication.

Original languageEnglish (US)
Pages (from-to)1419-1426
Number of pages8
JournalAlcoholism: Clinical and Experimental Research
Volume29
Issue number8
DOIs
StatePublished - Aug 2005

Fingerprint

Ataxia
Drinking
Ethanol
Self Administration
Blood
Brain
Foot
Alcoholic Intoxication
Ventral Tegmental Area
Gyrus Cinguli
Inbred C57BL Mouse
Drinking Water
Hippocampus

Keywords

  • Alcohol
  • Animal Model
  • Balance Beam
  • Mouse
  • Self-administration

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Ataxia and c-Fos expression in mice drinking ethanol in a limited access session. / Sharpe, Amanda L.; Tsivkovskaia, Natalia O.; Ryabinin, Andrey.

In: Alcoholism: Clinical and Experimental Research, Vol. 29, No. 8, 08.2005, p. 1419-1426.

Research output: Contribution to journalArticle

@article{ae138994125441399e5eb26f57efc084,
title = "Ataxia and c-Fos expression in mice drinking ethanol in a limited access session",
abstract = "Background: Although previous murine studies have demonstrated ethanol self-administration resulting in blood ethanol concentrations (BECs) believed to be pharmacologically relevant, to our knowledge, no study reported to date has demonstrated intoxication via ataxia after self-administration. Thus, the goal of this study was to demonstrate ataxia and to examine changes in c-Fos expression in mice after self-administration of intoxicating doses of ethanol. Methods: Male C57BL/6J mice were trained to drink a 10{\%} ethanol solution during daily 30-min limited access sessions. Mice were exposed to increasing concentrations of ethanol until a 10{\%} ethanol solution was reached. BEC and ataxia, measured as foot slips off of a balance beam, were examined after the limited access self-administration session. In a separate experiment, various brain structures from mice drinking water or ethanol were examined for changes in c-Fos expression two hr after the limited access session. Results: Mice drank between 1.5 and 2 g/kg of 10{\%} ethanol during the daily 30-min session. BECs for these mice 15 min after the limited access session ranged between 0.52 and 2.13 mg/ml. A significant increase in foot slips off a balance beam was seen immediately after ethanol consumption during the limited access session. Among mice drinking ethanol, an increase in c-Fos expression was seen in the Edinger-Westphal nucleus, and a decrease in c-Fos expression was seen in the cingulate cortex, ventral tegmental area, lateral and medial septum, CA1 region of the hippocampus, and basolateral amygdala. Conclusions: After this procedure in mice, BECs are achieved that are in a range considered pharmacologically relevant and intoxicating. Significant ataxia was observed after ethanol self-administration. Brain regions showing changes in c-Fos expression after voluntary intoxication were similar to those previously reported, suggesting that these brain regions are involved in regulating behavioral effects of alcohol intoxication.",
keywords = "Alcohol, Animal Model, Balance Beam, Mouse, Self-administration",
author = "Sharpe, {Amanda L.} and Tsivkovskaia, {Natalia O.} and Andrey Ryabinin",
year = "2005",
month = "8",
doi = "10.1097/01.alc.0000174746.64499.83",
language = "English (US)",
volume = "29",
pages = "1419--1426",
journal = "Alcoholism: Clinical and Experimental Research",
issn = "0145-6008",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Ataxia and c-Fos expression in mice drinking ethanol in a limited access session

AU - Sharpe, Amanda L.

AU - Tsivkovskaia, Natalia O.

AU - Ryabinin, Andrey

PY - 2005/8

Y1 - 2005/8

N2 - Background: Although previous murine studies have demonstrated ethanol self-administration resulting in blood ethanol concentrations (BECs) believed to be pharmacologically relevant, to our knowledge, no study reported to date has demonstrated intoxication via ataxia after self-administration. Thus, the goal of this study was to demonstrate ataxia and to examine changes in c-Fos expression in mice after self-administration of intoxicating doses of ethanol. Methods: Male C57BL/6J mice were trained to drink a 10% ethanol solution during daily 30-min limited access sessions. Mice were exposed to increasing concentrations of ethanol until a 10% ethanol solution was reached. BEC and ataxia, measured as foot slips off of a balance beam, were examined after the limited access self-administration session. In a separate experiment, various brain structures from mice drinking water or ethanol were examined for changes in c-Fos expression two hr after the limited access session. Results: Mice drank between 1.5 and 2 g/kg of 10% ethanol during the daily 30-min session. BECs for these mice 15 min after the limited access session ranged between 0.52 and 2.13 mg/ml. A significant increase in foot slips off a balance beam was seen immediately after ethanol consumption during the limited access session. Among mice drinking ethanol, an increase in c-Fos expression was seen in the Edinger-Westphal nucleus, and a decrease in c-Fos expression was seen in the cingulate cortex, ventral tegmental area, lateral and medial septum, CA1 region of the hippocampus, and basolateral amygdala. Conclusions: After this procedure in mice, BECs are achieved that are in a range considered pharmacologically relevant and intoxicating. Significant ataxia was observed after ethanol self-administration. Brain regions showing changes in c-Fos expression after voluntary intoxication were similar to those previously reported, suggesting that these brain regions are involved in regulating behavioral effects of alcohol intoxication.

AB - Background: Although previous murine studies have demonstrated ethanol self-administration resulting in blood ethanol concentrations (BECs) believed to be pharmacologically relevant, to our knowledge, no study reported to date has demonstrated intoxication via ataxia after self-administration. Thus, the goal of this study was to demonstrate ataxia and to examine changes in c-Fos expression in mice after self-administration of intoxicating doses of ethanol. Methods: Male C57BL/6J mice were trained to drink a 10% ethanol solution during daily 30-min limited access sessions. Mice were exposed to increasing concentrations of ethanol until a 10% ethanol solution was reached. BEC and ataxia, measured as foot slips off of a balance beam, were examined after the limited access self-administration session. In a separate experiment, various brain structures from mice drinking water or ethanol were examined for changes in c-Fos expression two hr after the limited access session. Results: Mice drank between 1.5 and 2 g/kg of 10% ethanol during the daily 30-min session. BECs for these mice 15 min after the limited access session ranged between 0.52 and 2.13 mg/ml. A significant increase in foot slips off a balance beam was seen immediately after ethanol consumption during the limited access session. Among mice drinking ethanol, an increase in c-Fos expression was seen in the Edinger-Westphal nucleus, and a decrease in c-Fos expression was seen in the cingulate cortex, ventral tegmental area, lateral and medial septum, CA1 region of the hippocampus, and basolateral amygdala. Conclusions: After this procedure in mice, BECs are achieved that are in a range considered pharmacologically relevant and intoxicating. Significant ataxia was observed after ethanol self-administration. Brain regions showing changes in c-Fos expression after voluntary intoxication were similar to those previously reported, suggesting that these brain regions are involved in regulating behavioral effects of alcohol intoxication.

KW - Alcohol

KW - Animal Model

KW - Balance Beam

KW - Mouse

KW - Self-administration

UR - http://www.scopus.com/inward/record.url?scp=24144440383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24144440383&partnerID=8YFLogxK

U2 - 10.1097/01.alc.0000174746.64499.83

DO - 10.1097/01.alc.0000174746.64499.83

M3 - Article

C2 - 16131849

AN - SCOPUS:24144440383

VL - 29

SP - 1419

EP - 1426

JO - Alcoholism: Clinical and Experimental Research

JF - Alcoholism: Clinical and Experimental Research

SN - 0145-6008

IS - 8

ER -