Asynchronous release of synaptic vesicles determines the time course of the AMPA receptor-mediated EPSC

Jeffrey S. Diamond; Craig E. Jahr

doi:10.1016/0896-6273(95)90098-5

Asynchronous release of synaptic vesicles determines the time course of the AMPA receptor-mediated EPSC

Jeffrey S. Diamond, Craig E. Jahr

Vollum Institute

Research output: Contribution to journal › Article › peer-review

263 Scopus citations

Abstract

The contribution of intersynaptic transmitter diffusion to the AMPA receptor EPSC time course was studied in cultured CA1 hippocampal neurons. Reducing release probability 20-fold with cadmium did not affect the time course of the averaged AMPA receptor EPSC, even when receptor desensitization was blocked by cyclothiazide, suggesting that individual synapses contribute independently to the AMPA receptor-mediated EPSC. Deconvolution of the averaged minlature EPSC from the evoked EPSC showed that release probability decays only slightly faster than the EPSC, suggesting that the AMPA receptor EPSC time course is determined primarily by the asynchrony of vesicle release. Further experiments demonstrated that cyclothiazide previously thought to affect only AMPA receptor kinetics, also enhances synaptic release.

Original language	English (US)
Pages (from-to)	1097-1107
Number of pages	11
Journal	Neuron
Volume	15
Issue number	5
DOIs	https://doi.org/10.1016/0896-6273(95)90098-5
State	Published - Nov 1995

ASJC Scopus subject areas

General Neuroscience

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10.1016/0896-6273(95)90098-5

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title = "Asynchronous release of synaptic vesicles determines the time course of the AMPA receptor-mediated EPSC",

abstract = "The contribution of intersynaptic transmitter diffusion to the AMPA receptor EPSC time course was studied in cultured CA1 hippocampal neurons. Reducing release probability 20-fold with cadmium did not affect the time course of the averaged AMPA receptor EPSC, even when receptor desensitization was blocked by cyclothiazide, suggesting that individual synapses contribute independently to the AMPA receptor-mediated EPSC. Deconvolution of the averaged minlature EPSC from the evoked EPSC showed that release probability decays only slightly faster than the EPSC, suggesting that the AMPA receptor EPSC time course is determined primarily by the asynchrony of vesicle release. Further experiments demonstrated that cyclothiazide previously thought to affect only AMPA receptor kinetics, also enhances synaptic release.",

author = "Diamond, {Jeffrey S.} and Jahr, {Craig E.}",

note = "Funding Information: We thank Mathew Jones and Indira Raman for discussions and comments on the manuscript, Jeffry Isaacson for helpful discussions, and Jeffrey Volk for preparing cell cultures. This work was supported by National Institutes of Health grants NS21419 (C. E. J.) and DK07680 (J. S. D.).",

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T1 - Asynchronous release of synaptic vesicles determines the time course of the AMPA receptor-mediated EPSC

AU - Diamond, Jeffrey S.

AU - Jahr, Craig E.

N1 - Funding Information: We thank Mathew Jones and Indira Raman for discussions and comments on the manuscript, Jeffry Isaacson for helpful discussions, and Jeffrey Volk for preparing cell cultures. This work was supported by National Institutes of Health grants NS21419 (C. E. J.) and DK07680 (J. S. D.).

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N2 - The contribution of intersynaptic transmitter diffusion to the AMPA receptor EPSC time course was studied in cultured CA1 hippocampal neurons. Reducing release probability 20-fold with cadmium did not affect the time course of the averaged AMPA receptor EPSC, even when receptor desensitization was blocked by cyclothiazide, suggesting that individual synapses contribute independently to the AMPA receptor-mediated EPSC. Deconvolution of the averaged minlature EPSC from the evoked EPSC showed that release probability decays only slightly faster than the EPSC, suggesting that the AMPA receptor EPSC time course is determined primarily by the asynchrony of vesicle release. Further experiments demonstrated that cyclothiazide previously thought to affect only AMPA receptor kinetics, also enhances synaptic release.

AB - The contribution of intersynaptic transmitter diffusion to the AMPA receptor EPSC time course was studied in cultured CA1 hippocampal neurons. Reducing release probability 20-fold with cadmium did not affect the time course of the averaged AMPA receptor EPSC, even when receptor desensitization was blocked by cyclothiazide, suggesting that individual synapses contribute independently to the AMPA receptor-mediated EPSC. Deconvolution of the averaged minlature EPSC from the evoked EPSC showed that release probability decays only slightly faster than the EPSC, suggesting that the AMPA receptor EPSC time course is determined primarily by the asynchrony of vesicle release. Further experiments demonstrated that cyclothiazide previously thought to affect only AMPA receptor kinetics, also enhances synaptic release.

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Asynchronous release of synaptic vesicles determines the time course of the AMPA receptor-mediated EPSC

Abstract

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