Asynchronous release of synaptic vesicles determines the time course of the AMPA receptor-mediated EPSC

Jeffrey S. Diamond, Craig E. Jahr

Research output: Contribution to journalArticle

246 Scopus citations

Abstract

The contribution of intersynaptic transmitter diffusion to the AMPA receptor EPSC time course was studied in cultured CA1 hippocampal neurons. Reducing release probability 20-fold with cadmium did not affect the time course of the averaged AMPA receptor EPSC, even when receptor desensitization was blocked by cyclothiazide, suggesting that individual synapses contribute independently to the AMPA receptor-mediated EPSC. Deconvolution of the averaged minlature EPSC from the evoked EPSC showed that release probability decays only slightly faster than the EPSC, suggesting that the AMPA receptor EPSC time course is determined primarily by the asynchrony of vesicle release. Further experiments demonstrated that cyclothiazide previously thought to affect only AMPA receptor kinetics, also enhances synaptic release.

Original languageEnglish (US)
Pages (from-to)1097-1107
Number of pages11
JournalNeuron
Volume15
Issue number5
DOIs
StatePublished - Nov 1995

ASJC Scopus subject areas

  • Neuroscience(all)

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