Association of p60c-src with polyoma virus middle-T antigen abrogating mitosis-specific activation

Stefanie Kaech-Petrie, Lidija Covic, Adrian Wyss, Kurt Ballmer-Hofer

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

POLYOMA middle-T antigen is required for tumorigenesis in animals and for viral transformation of a variety of cells in culture (reviewed in ref. 1). Middle-T associates with and thereby activates p60c-src, a cellular tyrosine kinase homologous to the oncogene product of Rous sarcoma virus2,3. Activation of p60c-src by middle-T is accompanied both by dephosphorylation of tyrosine 527, a site which negatively regulates src kinase activity (reviewed in refs 4-6) and by autophosphorylation on tyrosine 416 (refs 7-10). Phosphoprotein p60c-src is subject to cell cycle-specific regulation. It is most active during mitosis and repressed in interphase11. Here we report that mitotic p60c-src is dephosphorylated at tyrosine 527. We also show that in cells expressing middle-T, src kinase activity is high both in mitosis and during interphase. An oncogenic mutant src protein, p60c-src(527F), where tyrosine 527 is substituted by phenylalanine, is also highly active in all phases of the cell cycle.

Original languageEnglish (US)
Pages (from-to)431-433
Number of pages3
JournalNature
Volume350
Issue number6317
StatePublished - Apr 4 1991
Externally publishedYes

Fingerprint

Polyomavirus
Viral Tumor Antigens
Mitosis
Tyrosine
src-Family Kinases
Cell Cycle
Avian Sarcoma
Phosphoproteins
Oncogene Proteins
Interphase
Mutant Proteins
Phenylalanine
Protein-Tyrosine Kinases
Carcinogenesis
Cell Culture Techniques

ASJC Scopus subject areas

  • General

Cite this

Association of p60c-src with polyoma virus middle-T antigen abrogating mitosis-specific activation. / Kaech-Petrie, Stefanie; Covic, Lidija; Wyss, Adrian; Ballmer-Hofer, Kurt.

In: Nature, Vol. 350, No. 6317, 04.04.1991, p. 431-433.

Research output: Contribution to journalArticle

Kaech-Petrie, S, Covic, L, Wyss, A & Ballmer-Hofer, K 1991, 'Association of p60c-src with polyoma virus middle-T antigen abrogating mitosis-specific activation', Nature, vol. 350, no. 6317, pp. 431-433.
Kaech-Petrie, Stefanie ; Covic, Lidija ; Wyss, Adrian ; Ballmer-Hofer, Kurt. / Association of p60c-src with polyoma virus middle-T antigen abrogating mitosis-specific activation. In: Nature. 1991 ; Vol. 350, No. 6317. pp. 431-433.
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