Association of microtubule associated protein-2, synaptophysin, and apolipoprotein E mRNA and protein levels with cognition and anxiety levels in aged female rhesus macaques

Gwendolen E. Haley, Dominique H. Eghlidi, Steven Kohama, Henryk Urbanski, Jacob Raber

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The dendritic protein microtubule associated protein 2 (MAP-2), the presynaptic marker synaptophysin (SYN), and apolipoprotein E (APOE), a protein which plays a role in lipid transport and metabolism and affects synaptic activity show changes with age. We analyzed post-mortem tissue from aged female rhesus macaques cognitively tested in a spatial maze and classified as good spatial performers (GSP) or poor spatial performers (PSP) and behaviorally tested in a playroom and classified as bold or reserved animals. . MAP2, . SYN, and . APOE mRNA and protein levels in the prefrontal cortex (PFC), hippocampus, and amygdala, were assessed using qRT-PCR and western blot. In the amygdala, bold monkeys had higher levels of . MAP2 and . SYN mRNA than reserved monkeys. . MAP2 mRNA correlated positively with amygdala size on the right, left, and combined left and right sides, while . SYN mRNA levels correlated positively with the size of the right amygdala. In the hippocampus, SYN and APOE protein levels were higher in GSP than PSP animals. Thus, in aged nonhuman primates, classification of measures of anxiety is associated with differences in selected mRNA, but not protein, levels. In contrast, classification of cognitive performance is associated with differences in selected protein, but not mRNA, levels.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalBehavioural Brain Research
Volume232
Issue number1
DOIs
StatePublished - Jun 15 2012

Fingerprint

Synaptophysin
Microtubule-Associated Proteins
Apolipoproteins E
Macaca mulatta
Cognition
Anxiety
Amygdala
Messenger RNA
Proteins
Haplorhini
Hippocampus
Prefrontal Cortex
Lipid Metabolism
Primates
Western Blotting
Polymerase Chain Reaction

Keywords

  • APOE
  • Cognitive function
  • MAP-2
  • SYN

ASJC Scopus subject areas

  • Behavioral Neuroscience

Cite this

Association of microtubule associated protein-2, synaptophysin, and apolipoprotein E mRNA and protein levels with cognition and anxiety levels in aged female rhesus macaques. / Haley, Gwendolen E.; Eghlidi, Dominique H.; Kohama, Steven; Urbanski, Henryk; Raber, Jacob.

In: Behavioural Brain Research, Vol. 232, No. 1, 15.06.2012, p. 1-6.

Research output: Contribution to journalArticle

@article{15bcf9f7e67b461b9df2ad7bb9173031,
title = "Association of microtubule associated protein-2, synaptophysin, and apolipoprotein E mRNA and protein levels with cognition and anxiety levels in aged female rhesus macaques",
abstract = "The dendritic protein microtubule associated protein 2 (MAP-2), the presynaptic marker synaptophysin (SYN), and apolipoprotein E (APOE), a protein which plays a role in lipid transport and metabolism and affects synaptic activity show changes with age. We analyzed post-mortem tissue from aged female rhesus macaques cognitively tested in a spatial maze and classified as good spatial performers (GSP) or poor spatial performers (PSP) and behaviorally tested in a playroom and classified as bold or reserved animals. . MAP2, . SYN, and . APOE mRNA and protein levels in the prefrontal cortex (PFC), hippocampus, and amygdala, were assessed using qRT-PCR and western blot. In the amygdala, bold monkeys had higher levels of . MAP2 and . SYN mRNA than reserved monkeys. . MAP2 mRNA correlated positively with amygdala size on the right, left, and combined left and right sides, while . SYN mRNA levels correlated positively with the size of the right amygdala. In the hippocampus, SYN and APOE protein levels were higher in GSP than PSP animals. Thus, in aged nonhuman primates, classification of measures of anxiety is associated with differences in selected mRNA, but not protein, levels. In contrast, classification of cognitive performance is associated with differences in selected protein, but not mRNA, levels.",
keywords = "APOE, Cognitive function, MAP-2, SYN",
author = "Haley, {Gwendolen E.} and Eghlidi, {Dominique H.} and Steven Kohama and Henryk Urbanski and Jacob Raber",
year = "2012",
month = "6",
day = "15",
doi = "10.1016/j.bbr.2012.03.032",
language = "English (US)",
volume = "232",
pages = "1--6",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Association of microtubule associated protein-2, synaptophysin, and apolipoprotein E mRNA and protein levels with cognition and anxiety levels in aged female rhesus macaques

AU - Haley, Gwendolen E.

AU - Eghlidi, Dominique H.

AU - Kohama, Steven

AU - Urbanski, Henryk

AU - Raber, Jacob

PY - 2012/6/15

Y1 - 2012/6/15

N2 - The dendritic protein microtubule associated protein 2 (MAP-2), the presynaptic marker synaptophysin (SYN), and apolipoprotein E (APOE), a protein which plays a role in lipid transport and metabolism and affects synaptic activity show changes with age. We analyzed post-mortem tissue from aged female rhesus macaques cognitively tested in a spatial maze and classified as good spatial performers (GSP) or poor spatial performers (PSP) and behaviorally tested in a playroom and classified as bold or reserved animals. . MAP2, . SYN, and . APOE mRNA and protein levels in the prefrontal cortex (PFC), hippocampus, and amygdala, were assessed using qRT-PCR and western blot. In the amygdala, bold monkeys had higher levels of . MAP2 and . SYN mRNA than reserved monkeys. . MAP2 mRNA correlated positively with amygdala size on the right, left, and combined left and right sides, while . SYN mRNA levels correlated positively with the size of the right amygdala. In the hippocampus, SYN and APOE protein levels were higher in GSP than PSP animals. Thus, in aged nonhuman primates, classification of measures of anxiety is associated with differences in selected mRNA, but not protein, levels. In contrast, classification of cognitive performance is associated with differences in selected protein, but not mRNA, levels.

AB - The dendritic protein microtubule associated protein 2 (MAP-2), the presynaptic marker synaptophysin (SYN), and apolipoprotein E (APOE), a protein which plays a role in lipid transport and metabolism and affects synaptic activity show changes with age. We analyzed post-mortem tissue from aged female rhesus macaques cognitively tested in a spatial maze and classified as good spatial performers (GSP) or poor spatial performers (PSP) and behaviorally tested in a playroom and classified as bold or reserved animals. . MAP2, . SYN, and . APOE mRNA and protein levels in the prefrontal cortex (PFC), hippocampus, and amygdala, were assessed using qRT-PCR and western blot. In the amygdala, bold monkeys had higher levels of . MAP2 and . SYN mRNA than reserved monkeys. . MAP2 mRNA correlated positively with amygdala size on the right, left, and combined left and right sides, while . SYN mRNA levels correlated positively with the size of the right amygdala. In the hippocampus, SYN and APOE protein levels were higher in GSP than PSP animals. Thus, in aged nonhuman primates, classification of measures of anxiety is associated with differences in selected mRNA, but not protein, levels. In contrast, classification of cognitive performance is associated with differences in selected protein, but not mRNA, levels.

KW - APOE

KW - Cognitive function

KW - MAP-2

KW - SYN

UR - http://www.scopus.com/inward/record.url?scp=84859786257&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859786257&partnerID=8YFLogxK

U2 - 10.1016/j.bbr.2012.03.032

DO - 10.1016/j.bbr.2012.03.032

M3 - Article

C2 - 22475553

AN - SCOPUS:84859786257

VL - 232

SP - 1

EP - 6

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

IS - 1

ER -