The majority of melanoma tumor antigen activity present in melanoma extracts derived from fresh tumor tissue binds to a Sepharose-anti-β2-microglobulin adsorbent. Removal of HLA antigens from the extracts of melanoma tissue by using a KBr flotation technique did not reduce either the tumor antigen activity of the extracts or the binding of melanoma tumor antigen (MTA) activity to the Sepharose-anti-β2-microglobulin adsorbent. The complete blocking of MTA activity by pretreating the anti-β2-microglobulin adsorbent with β2-microglobulin and the lack of detectable MTA binding to a Sepharose anti-normal human serum adsorbent demonstrated the specificity of the binding of MTA to the anti-β2-microglobulin adsorbent.
|Original language||English (US)|
|Number of pages||5|
|Issue number||2 II|
|State||Published - Oct 3 1979|
ASJC Scopus subject areas
- Cancer Research