Association of human transferrin receptor with GABARAP

Frank Green, Thomas O'Hare, Aaron Blackwell, Caroline Enns

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A yeast two-hybrid screen identified a specific interaction between the cytoplasmic domain of transferrin receptor (TfR) and GABARAP, a 14 kDa protein that binds to the γ2 subunit of neuronal GABAA receptors. The specificity of the TfR-GABARAP interaction was demonstrated by in vitro binding assays with purified proteins and by co-immunoprecipitation of GABARAP with endogenous TfR from HeLa cell lysates. Replacement of the YTRF internalization motif with ATRA within the cytoplasmic domain of TfR reduced interaction with GABARAP in the yeast two-hybrid screen and in vitro binding assays. The intracellular location of GABARAP using chimeric GABARAP-GFP showed that the majority of GABARAP was located in perinuclear vesicles. Our results show that GABARAP plays a more general role outside the confines of neuronal cells and GABAA receptors.

Original languageEnglish (US)
Pages (from-to)101-106
Number of pages6
JournalFEBS Letters
Volume518
Issue number1-3
DOIs
StatePublished - May 8 2002

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Transferrin Receptors
GABA-A Receptors
Yeast
Assays
Yeasts
HeLa Cells
Immunoprecipitation
Proteins
In Vitro Techniques

Keywords

  • γ-Aminobutyric acid type A receptor-associated protein
  • Endocytic motif
  • Protein trafficking
  • Transferrin receptor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Association of human transferrin receptor with GABARAP. / Green, Frank; O'Hare, Thomas; Blackwell, Aaron; Enns, Caroline.

In: FEBS Letters, Vol. 518, No. 1-3, 08.05.2002, p. 101-106.

Research output: Contribution to journalArticle

Green, Frank ; O'Hare, Thomas ; Blackwell, Aaron ; Enns, Caroline. / Association of human transferrin receptor with GABARAP. In: FEBS Letters. 2002 ; Vol. 518, No. 1-3. pp. 101-106.
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