Association of genetic variations in aromatase gene with serum estrogen and estrogen/testosterone ratio in Chinese elderly men

Jieying Jiang, Nelson L S Tang, C. Ohlsson, Anna L. Eriksson, Liesbeth Vandenput, Frank W K Chan, Jasmine K L Ching, Anthony Kwok, Eric Orwoll, Timothy C Y Kwok, Jean Woo, Ping Chung Leung

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Single nucleotide polymorphism (SNP) rs2470152 of the gene CYP19A1 is associated with serum estradiol (E2) levels in Caucasian men. However, it remains to be verified if rs2470152 is the sole determinant accounting for the association. We determined whether 2 CYP19A1 SNPs tagging different haploblocks (rs2470152 and rs2899470) are associated with sex steroid levels in Chinese men. Method: Serum sex steroid level including E2, estrone (E1) and testosterone (T), of 1402 Chinese men aged ≥ 65 years were analyzed. Genotyping of the two CYP19A1 SNPs was performed using Tm-shift allele-specific PCR. Results: SNP rs2899470 was significantly associated with serum E2, E1 levels and E2/T ratio (p <0.001). However, SNP rs2470152 was only modestly associated with E2/T ratio (p = 0.023). Analysis of haplotype showed a significant association between C-G, T-T haplotype with serum E2/T ratio (p = 0.019 and p = 1 × 10- 5, respectively). Similarly, E2 levels was also associated the T-T and T-G haplotypes (p = 1 × 10- 5). Conclusion: The genetic variation of CYP19A1 was associated with circulating estrogen levels in Chinese elderly men. In addition, it revealed that haplotype of rs2899470 and rs2470152, rather than rs2899470 alone, was a better indicator for the serum E2/T ratio and E2 levels.

Original languageEnglish (US)
Pages (from-to)53-58
Number of pages6
JournalClinica Chimica Acta
Volume411
Issue number1-2
DOIs
StatePublished - Jan 4 2010

Fingerprint

Aromatase
Polymorphism
Single Nucleotide Polymorphism
Testosterone
Estrogens
Nucleotides
Genes
Haplotypes
Serum
Steroids
Estrone
Estradiol
Alleles
Polymerase Chain Reaction

Keywords

  • CYP19A1
  • Sex steroid levels
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Jiang, J., Tang, N. L. S., Ohlsson, C., Eriksson, A. L., Vandenput, L., Chan, F. W. K., ... Leung, P. C. (2010). Association of genetic variations in aromatase gene with serum estrogen and estrogen/testosterone ratio in Chinese elderly men. Clinica Chimica Acta, 411(1-2), 53-58. https://doi.org/10.1016/j.cca.2009.09.039

Association of genetic variations in aromatase gene with serum estrogen and estrogen/testosterone ratio in Chinese elderly men. / Jiang, Jieying; Tang, Nelson L S; Ohlsson, C.; Eriksson, Anna L.; Vandenput, Liesbeth; Chan, Frank W K; Ching, Jasmine K L; Kwok, Anthony; Orwoll, Eric; Kwok, Timothy C Y; Woo, Jean; Leung, Ping Chung.

In: Clinica Chimica Acta, Vol. 411, No. 1-2, 04.01.2010, p. 53-58.

Research output: Contribution to journalArticle

Jiang, J, Tang, NLS, Ohlsson, C, Eriksson, AL, Vandenput, L, Chan, FWK, Ching, JKL, Kwok, A, Orwoll, E, Kwok, TCY, Woo, J & Leung, PC 2010, 'Association of genetic variations in aromatase gene with serum estrogen and estrogen/testosterone ratio in Chinese elderly men', Clinica Chimica Acta, vol. 411, no. 1-2, pp. 53-58. https://doi.org/10.1016/j.cca.2009.09.039
Jiang, Jieying ; Tang, Nelson L S ; Ohlsson, C. ; Eriksson, Anna L. ; Vandenput, Liesbeth ; Chan, Frank W K ; Ching, Jasmine K L ; Kwok, Anthony ; Orwoll, Eric ; Kwok, Timothy C Y ; Woo, Jean ; Leung, Ping Chung. / Association of genetic variations in aromatase gene with serum estrogen and estrogen/testosterone ratio in Chinese elderly men. In: Clinica Chimica Acta. 2010 ; Vol. 411, No. 1-2. pp. 53-58.
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title = "Association of genetic variations in aromatase gene with serum estrogen and estrogen/testosterone ratio in Chinese elderly men",
abstract = "Background: Single nucleotide polymorphism (SNP) rs2470152 of the gene CYP19A1 is associated with serum estradiol (E2) levels in Caucasian men. However, it remains to be verified if rs2470152 is the sole determinant accounting for the association. We determined whether 2 CYP19A1 SNPs tagging different haploblocks (rs2470152 and rs2899470) are associated with sex steroid levels in Chinese men. Method: Serum sex steroid level including E2, estrone (E1) and testosterone (T), of 1402 Chinese men aged ≥ 65 years were analyzed. Genotyping of the two CYP19A1 SNPs was performed using Tm-shift allele-specific PCR. Results: SNP rs2899470 was significantly associated with serum E2, E1 levels and E2/T ratio (p <0.001). However, SNP rs2470152 was only modestly associated with E2/T ratio (p = 0.023). Analysis of haplotype showed a significant association between C-G, T-T haplotype with serum E2/T ratio (p = 0.019 and p = 1 × 10- 5, respectively). Similarly, E2 levels was also associated the T-T and T-G haplotypes (p = 1 × 10- 5). Conclusion: The genetic variation of CYP19A1 was associated with circulating estrogen levels in Chinese elderly men. In addition, it revealed that haplotype of rs2899470 and rs2470152, rather than rs2899470 alone, was a better indicator for the serum E2/T ratio and E2 levels.",
keywords = "CYP19A1, Sex steroid levels, Single nucleotide polymorphism",
author = "Jieying Jiang and Tang, {Nelson L S} and C. Ohlsson and Eriksson, {Anna L.} and Liesbeth Vandenput and Chan, {Frank W K} and Ching, {Jasmine K L} and Anthony Kwok and Eric Orwoll and Kwok, {Timothy C Y} and Jean Woo and Leung, {Ping Chung}",
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T1 - Association of genetic variations in aromatase gene with serum estrogen and estrogen/testosterone ratio in Chinese elderly men

AU - Jiang, Jieying

AU - Tang, Nelson L S

AU - Ohlsson, C.

AU - Eriksson, Anna L.

AU - Vandenput, Liesbeth

AU - Chan, Frank W K

AU - Ching, Jasmine K L

AU - Kwok, Anthony

AU - Orwoll, Eric

AU - Kwok, Timothy C Y

AU - Woo, Jean

AU - Leung, Ping Chung

PY - 2010/1/4

Y1 - 2010/1/4

N2 - Background: Single nucleotide polymorphism (SNP) rs2470152 of the gene CYP19A1 is associated with serum estradiol (E2) levels in Caucasian men. However, it remains to be verified if rs2470152 is the sole determinant accounting for the association. We determined whether 2 CYP19A1 SNPs tagging different haploblocks (rs2470152 and rs2899470) are associated with sex steroid levels in Chinese men. Method: Serum sex steroid level including E2, estrone (E1) and testosterone (T), of 1402 Chinese men aged ≥ 65 years were analyzed. Genotyping of the two CYP19A1 SNPs was performed using Tm-shift allele-specific PCR. Results: SNP rs2899470 was significantly associated with serum E2, E1 levels and E2/T ratio (p <0.001). However, SNP rs2470152 was only modestly associated with E2/T ratio (p = 0.023). Analysis of haplotype showed a significant association between C-G, T-T haplotype with serum E2/T ratio (p = 0.019 and p = 1 × 10- 5, respectively). Similarly, E2 levels was also associated the T-T and T-G haplotypes (p = 1 × 10- 5). Conclusion: The genetic variation of CYP19A1 was associated with circulating estrogen levels in Chinese elderly men. In addition, it revealed that haplotype of rs2899470 and rs2470152, rather than rs2899470 alone, was a better indicator for the serum E2/T ratio and E2 levels.

AB - Background: Single nucleotide polymorphism (SNP) rs2470152 of the gene CYP19A1 is associated with serum estradiol (E2) levels in Caucasian men. However, it remains to be verified if rs2470152 is the sole determinant accounting for the association. We determined whether 2 CYP19A1 SNPs tagging different haploblocks (rs2470152 and rs2899470) are associated with sex steroid levels in Chinese men. Method: Serum sex steroid level including E2, estrone (E1) and testosterone (T), of 1402 Chinese men aged ≥ 65 years were analyzed. Genotyping of the two CYP19A1 SNPs was performed using Tm-shift allele-specific PCR. Results: SNP rs2899470 was significantly associated with serum E2, E1 levels and E2/T ratio (p <0.001). However, SNP rs2470152 was only modestly associated with E2/T ratio (p = 0.023). Analysis of haplotype showed a significant association between C-G, T-T haplotype with serum E2/T ratio (p = 0.019 and p = 1 × 10- 5, respectively). Similarly, E2 levels was also associated the T-T and T-G haplotypes (p = 1 × 10- 5). Conclusion: The genetic variation of CYP19A1 was associated with circulating estrogen levels in Chinese elderly men. In addition, it revealed that haplotype of rs2899470 and rs2470152, rather than rs2899470 alone, was a better indicator for the serum E2/T ratio and E2 levels.

KW - CYP19A1

KW - Sex steroid levels

KW - Single nucleotide polymorphism

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