Association of dopamine D3 receptors with actin-binding protein 280 (ABP-280)

Ming Li, Chuanyu Li, Paul Weingarten, James R. Bunzow, David K. Grandy, Qun Yong Zhou

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Proteins that bind to G protein-coupled receptors have been identified as regulators of receptor localization and signaling. In our previous studies, a cytoskeletal protein, actin-binding protein 280 (ABP-280), was found to associate with the third cytoplasmic loop of dopamine D2 receptors. In this study, we demonstrate that ABP-280 also interacts with dopamine D3 receptors, but not with D4 receptors. Similar to the dopamine D2 receptor, the D3/ABP-280 association is of signaling importance. In human melanoma M2 cells lacking ABP-280, D3 receptors were unable to inhibit forskolin-stimulated cyclic AMP (cAMP) production significantly. D4 receptors, however, exhibited a similar degree of inhibition of forskolin-stimulated cAMP production in ABP-280-deficient M2 cells and ABP-280-replent M2 subclones (A7 cells). Further experiments revealed that the D3/ABP-280 interaction was critically dependent upon a 36 amino acid carboxyl domain of the D3 receptor third loop, which is conserved in the D2 receptor but not in the D4 receptor. Our results demonstrate a subtype-specific regulation of dopamine D2-family receptor signaling by the cytoskeletal protein ABP-280.

Original languageEnglish (US)
Pages (from-to)859-863
Number of pages5
JournalBiochemical Pharmacology
Issue number5
StatePublished - Mar 1 2002
Externally publishedYes


  • Actin-binding protein 280
  • Dopamine
  • G protein-coupled receptor
  • Protein interaction
  • Receptor subtypes
  • Signaling

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology


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