Association of dopamine D3 receptors with actin-binding protein 280 (ABP-280)

Ming Li, Chuanyu Li, Paul Weingarten, James R. Bunzow, David Grandy, Qun Yong Zhou

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    Proteins that bind to G protein-coupled receptors have been identified as regulators of receptor localization and signaling. In our previous studies, a cytoskeletal protein, actin-binding protein 280 (ABP-280), was found to associate with the third cytoplasmic loop of dopamine D2 receptors. In this study, we demonstrate that ABP-280 also interacts with dopamine D3 receptors, but not with D4 receptors. Similar to the dopamine D2 receptor, the D3/ABP-280 association is of signaling importance. In human melanoma M2 cells lacking ABP-280, D3 receptors were unable to inhibit forskolin-stimulated cyclic AMP (cAMP) production significantly. D4 receptors, however, exhibited a similar degree of inhibition of forskolin-stimulated cAMP production in ABP-280-deficient M2 cells and ABP-280-replent M2 subclones (A7 cells). Further experiments revealed that the D3/ABP-280 interaction was critically dependent upon a 36 amino acid carboxyl domain of the D3 receptor third loop, which is conserved in the D2 receptor but not in the D4 receptor. Our results demonstrate a subtype-specific regulation of dopamine D2-family receptor signaling by the cytoskeletal protein ABP-280.

    Original languageEnglish (US)
    Pages (from-to)859-863
    Number of pages5
    JournalBiochemical Pharmacology
    Volume63
    Issue number5
    DOIs
    StatePublished - Mar 1 2002

    Fingerprint

    Dopamine D3 Receptors
    Microfilament Proteins
    Dopamine D2 Receptors
    Cytoskeletal Proteins
    Colforsin
    Protein Binding
    Cyclic AMP
    G-Protein-Coupled Receptors
    Melanoma
    Dopamine
    Amino Acids

    Keywords

    • Actin-binding protein 280
    • Dopamine
    • G protein-coupled receptor
    • Protein interaction
    • Receptor subtypes
    • Signaling

    ASJC Scopus subject areas

    • Pharmacology

    Cite this

    Li, M., Li, C., Weingarten, P., Bunzow, J. R., Grandy, D., & Zhou, Q. Y. (2002). Association of dopamine D3 receptors with actin-binding protein 280 (ABP-280). Biochemical Pharmacology, 63(5), 859-863. https://doi.org/10.1016/S0006-2952(01)00932-7

    Association of dopamine D3 receptors with actin-binding protein 280 (ABP-280). / Li, Ming; Li, Chuanyu; Weingarten, Paul; Bunzow, James R.; Grandy, David; Zhou, Qun Yong.

    In: Biochemical Pharmacology, Vol. 63, No. 5, 01.03.2002, p. 859-863.

    Research output: Contribution to journalArticle

    Li, M, Li, C, Weingarten, P, Bunzow, JR, Grandy, D & Zhou, QY 2002, 'Association of dopamine D3 receptors with actin-binding protein 280 (ABP-280)', Biochemical Pharmacology, vol. 63, no. 5, pp. 859-863. https://doi.org/10.1016/S0006-2952(01)00932-7
    Li, Ming ; Li, Chuanyu ; Weingarten, Paul ; Bunzow, James R. ; Grandy, David ; Zhou, Qun Yong. / Association of dopamine D3 receptors with actin-binding protein 280 (ABP-280). In: Biochemical Pharmacology. 2002 ; Vol. 63, No. 5. pp. 859-863.
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