Association of black-pigmented bacteria with endodontic infections

John (Craig) Baumgartner, Beverly J. Watkins, Kwang Shik Bae, Tian Xia

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Black-pigmented bacteria (BPB) have been associated with endodontic infections. The purpose of this study was to evaluate further the presence of BPB with the clinical signs and symptoms associated with endodontic infections. Microbial samples were collected from the root canals of 40 intact teeth with necrotic pulps and apical periodontitis. Conventional laboratory methods were used for identification of the strains of BPB isolated in pure culture. In addition, the polymerase chain reaction and specific primers for 16S r-RNA genes were used to differentiate Prevotella nigrescens from Prevotella intermedia. Twenty-two (55%) samples were positive for the growth of BPB. Of those, 11 of 22 (50%) were identified as P. nigrescens, 8 of 22 (36%) were P. intermedia, 2 of 22 (9%) were Porphyromonas gingivalis, and 1 of 22 (5%) was Prevotella melaninogenica. Sixteen of the 22 root canals positive for the growth of BPB were associated with purulent drainage either from the root canal or an associated sinus tract. Statistical analysis did not show a significant relationship for the presence of BPB with clinical signs and symptoms.

Original languageEnglish (US)
Pages (from-to)413-415
Number of pages3
JournalJournal of Endodontics
Volume25
Issue number6
StatePublished - Jun 1999

Fingerprint

Endodontics
Bacteria
Dental Pulp Cavity
Prevotella nigrescens
Infection
Prevotella intermedia
Signs and Symptoms
Prevotella melaninogenica
Periapical Periodontitis
Porphyromonas gingivalis
Growth
Drainage
Tooth
RNA
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Association of black-pigmented bacteria with endodontic infections. / Baumgartner, John (Craig); Watkins, Beverly J.; Bae, Kwang Shik; Xia, Tian.

In: Journal of Endodontics, Vol. 25, No. 6, 06.1999, p. 413-415.

Research output: Contribution to journalArticle

Baumgartner, JC, Watkins, BJ, Bae, KS & Xia, T 1999, 'Association of black-pigmented bacteria with endodontic infections', Journal of Endodontics, vol. 25, no. 6, pp. 413-415.
Baumgartner, John (Craig) ; Watkins, Beverly J. ; Bae, Kwang Shik ; Xia, Tian. / Association of black-pigmented bacteria with endodontic infections. In: Journal of Endodontics. 1999 ; Vol. 25, No. 6. pp. 413-415.
@article{f74cbd8f8cd5441186fe394157ffbeea,
title = "Association of black-pigmented bacteria with endodontic infections",
abstract = "Black-pigmented bacteria (BPB) have been associated with endodontic infections. The purpose of this study was to evaluate further the presence of BPB with the clinical signs and symptoms associated with endodontic infections. Microbial samples were collected from the root canals of 40 intact teeth with necrotic pulps and apical periodontitis. Conventional laboratory methods were used for identification of the strains of BPB isolated in pure culture. In addition, the polymerase chain reaction and specific primers for 16S r-RNA genes were used to differentiate Prevotella nigrescens from Prevotella intermedia. Twenty-two (55{\%}) samples were positive for the growth of BPB. Of those, 11 of 22 (50{\%}) were identified as P. nigrescens, 8 of 22 (36{\%}) were P. intermedia, 2 of 22 (9{\%}) were Porphyromonas gingivalis, and 1 of 22 (5{\%}) was Prevotella melaninogenica. Sixteen of the 22 root canals positive for the growth of BPB were associated with purulent drainage either from the root canal or an associated sinus tract. Statistical analysis did not show a significant relationship for the presence of BPB with clinical signs and symptoms.",
author = "Baumgartner, {John (Craig)} and Watkins, {Beverly J.} and Bae, {Kwang Shik} and Tian Xia",
year = "1999",
month = "6",
language = "English (US)",
volume = "25",
pages = "413--415",
journal = "Journal of Endodontics",
issn = "0099-2399",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Association of black-pigmented bacteria with endodontic infections

AU - Baumgartner, John (Craig)

AU - Watkins, Beverly J.

AU - Bae, Kwang Shik

AU - Xia, Tian

PY - 1999/6

Y1 - 1999/6

N2 - Black-pigmented bacteria (BPB) have been associated with endodontic infections. The purpose of this study was to evaluate further the presence of BPB with the clinical signs and symptoms associated with endodontic infections. Microbial samples were collected from the root canals of 40 intact teeth with necrotic pulps and apical periodontitis. Conventional laboratory methods were used for identification of the strains of BPB isolated in pure culture. In addition, the polymerase chain reaction and specific primers for 16S r-RNA genes were used to differentiate Prevotella nigrescens from Prevotella intermedia. Twenty-two (55%) samples were positive for the growth of BPB. Of those, 11 of 22 (50%) were identified as P. nigrescens, 8 of 22 (36%) were P. intermedia, 2 of 22 (9%) were Porphyromonas gingivalis, and 1 of 22 (5%) was Prevotella melaninogenica. Sixteen of the 22 root canals positive for the growth of BPB were associated with purulent drainage either from the root canal or an associated sinus tract. Statistical analysis did not show a significant relationship for the presence of BPB with clinical signs and symptoms.

AB - Black-pigmented bacteria (BPB) have been associated with endodontic infections. The purpose of this study was to evaluate further the presence of BPB with the clinical signs and symptoms associated with endodontic infections. Microbial samples were collected from the root canals of 40 intact teeth with necrotic pulps and apical periodontitis. Conventional laboratory methods were used for identification of the strains of BPB isolated in pure culture. In addition, the polymerase chain reaction and specific primers for 16S r-RNA genes were used to differentiate Prevotella nigrescens from Prevotella intermedia. Twenty-two (55%) samples were positive for the growth of BPB. Of those, 11 of 22 (50%) were identified as P. nigrescens, 8 of 22 (36%) were P. intermedia, 2 of 22 (9%) were Porphyromonas gingivalis, and 1 of 22 (5%) was Prevotella melaninogenica. Sixteen of the 22 root canals positive for the growth of BPB were associated with purulent drainage either from the root canal or an associated sinus tract. Statistical analysis did not show a significant relationship for the presence of BPB with clinical signs and symptoms.

UR - http://www.scopus.com/inward/record.url?scp=0033147252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033147252&partnerID=8YFLogxK

M3 - Article

C2 - 10530240

AN - SCOPUS:0033147252

VL - 25

SP - 413

EP - 415

JO - Journal of Endodontics

JF - Journal of Endodontics

SN - 0099-2399

IS - 6

ER -