Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3

Gregory D. Plowman, Joseph P. Brown, Caroline Enns, Jim Schröder, Barbro Nikinmaa, Howard H. Sussman, Karl Erik Hellström, Ingegerd Hellström

Research output: Contribution to journalArticle

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Abstract

p97 is a 97,000 molecular weight cell-surface glycoprotein, which is present in human melanomas but in only trace amounts in normal adult tissues1-4. Amino acid sequence and iron binding studies have shown that p97 is structurally and functionally related to transferrin5. Reports that the genes for the transferrin receptor (TR)6-8 and possibly transferrin9-13 are located on chromosome 3 prompted us to investigate the chromosomal localization of the p97 gene. Our strategy was to characterize interspecies somatic cell hybrids derived from human fibroblasts or lymphocytes for expression of p97 and presence of human chromosomes. Although fibroblasts and lymphocytes express only small amounts of p973,4, we were able to type the hybrids for p97 by using monoclonal antibodies in highly sensitive and specific immunoassays. Of 14 hybrids, 6 contained chromosome 3 and expressed p97, and 8 were negative for both. We conclude that the p97 gene, like the transferrin and TR genes, is located on chromosome 3.

Original languageEnglish (US)
Pages (from-to)70-72
Number of pages3
JournalNature
Volume303
Issue number5912
DOIs
StatePublished - 1983
Externally publishedYes

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Chromosomes, Human, Pair 3
Transferrin Receptors
Genes
Fibroblasts
Lymphocytes
Hybrid Cells
Membrane Glycoproteins
Human Chromosomes
Transferrin
Immunoassay
Amino Acid Sequence
Melanoma
Iron
Molecular Weight
Monoclonal Antibodies
human melanotransferrin protein

ASJC Scopus subject areas

  • General

Cite this

Plowman, G. D., Brown, J. P., Enns, C., Schröder, J., Nikinmaa, B., Sussman, H. H., ... Hellström, I. (1983). Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3. Nature, 303(5912), 70-72. https://doi.org/10.1038/303070a0

Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3. / Plowman, Gregory D.; Brown, Joseph P.; Enns, Caroline; Schröder, Jim; Nikinmaa, Barbro; Sussman, Howard H.; Hellström, Karl Erik; Hellström, Ingegerd.

In: Nature, Vol. 303, No. 5912, 1983, p. 70-72.

Research output: Contribution to journalArticle

Plowman, GD, Brown, JP, Enns, C, Schröder, J, Nikinmaa, B, Sussman, HH, Hellström, KE & Hellström, I 1983, 'Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3', Nature, vol. 303, no. 5912, pp. 70-72. https://doi.org/10.1038/303070a0
Plowman GD, Brown JP, Enns C, Schröder J, Nikinmaa B, Sussman HH et al. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3. Nature. 1983;303(5912):70-72. https://doi.org/10.1038/303070a0
Plowman, Gregory D. ; Brown, Joseph P. ; Enns, Caroline ; Schröder, Jim ; Nikinmaa, Barbro ; Sussman, Howard H. ; Hellström, Karl Erik ; Hellström, Ingegerd. / Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3. In: Nature. 1983 ; Vol. 303, No. 5912. pp. 70-72.
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