Assessment of Ischemia-Induced Microvascular Remodeling Using Contrast-Enhanced Ultrasound Vascular Anatomic Mapping

Marco Pascotto, Howard Leong-Poi, Beat Kaufmann, Achim Allrogen, Dimitrios Charalampidis, Edmund K. Kerut, Sanjiv Kaul, Jonathan Lindner

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Our aim was to apply novel contrast-enhanced ultrasound (CEU) techniques to characterize remodeling in different vascular compartments during ischemia-mediated angiogenesis. Hind limb ischemia was produced by ligation of an external iliac artery in 60 rats, half of which were treated with intramuscular fibroblast growth factor (FGF)-2 (5 μg). The proximal adductor muscles of the ischemic and control hind limb were studied immediately after ligation and at days 4, 7, or 14. Low-power maximum intensity projection imaging was performed to assess large intramuscular vessels to the fourth branch order. CEU data were analyzed to measure capillary perfusion and functional noncapillary microvascular blood volume. Resting capillary perfusion was reduced by 30% after arterial ligation and recovered earlier in FGF-2-treated versus nontreated rats (day 4 vs. 14). Changes in perfusion were temporally related to expansion of noncapillary microvascular blood volume on CEU, which was associated with an arteriogenic response on histology. Expansion of and organization (fractal distribution) of large collateral vessels occurred gradually over 2 weeks and was slightly more rapid with FGF-2 treatment. We conclude that CEU can separately assess collateral development, more distal arteriogenesis, and secondary changes in capillary perfusion that occur differentially with ischemia and growth factor therapy.

    Original languageEnglish (US)
    Pages (from-to)1100-1108
    Number of pages9
    JournalJournal of the American Society of Echocardiography
    Volume20
    Issue number9
    DOIs
    StatePublished - Sep 2007

    Fingerprint

    Blood Vessels
    Fibroblast Growth Factor 2
    Ischemia
    Perfusion
    Ligation
    Blood Volume
    Fibroblast Growth Factor 5
    Extremities
    Fractals
    Iliac Artery
    Intercellular Signaling Peptides and Proteins
    Histology
    Muscles
    Therapeutics

    ASJC Scopus subject areas

    • Radiology Nuclear Medicine and imaging
    • Cardiology and Cardiovascular Medicine

    Cite this

    Assessment of Ischemia-Induced Microvascular Remodeling Using Contrast-Enhanced Ultrasound Vascular Anatomic Mapping. / Pascotto, Marco; Leong-Poi, Howard; Kaufmann, Beat; Allrogen, Achim; Charalampidis, Dimitrios; Kerut, Edmund K.; Kaul, Sanjiv; Lindner, Jonathan.

    In: Journal of the American Society of Echocardiography, Vol. 20, No. 9, 09.2007, p. 1100-1108.

    Research output: Contribution to journalArticle

    Pascotto, Marco ; Leong-Poi, Howard ; Kaufmann, Beat ; Allrogen, Achim ; Charalampidis, Dimitrios ; Kerut, Edmund K. ; Kaul, Sanjiv ; Lindner, Jonathan. / Assessment of Ischemia-Induced Microvascular Remodeling Using Contrast-Enhanced Ultrasound Vascular Anatomic Mapping. In: Journal of the American Society of Echocardiography. 2007 ; Vol. 20, No. 9. pp. 1100-1108.
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