Assessment of GABA-B, metabotropic glutamate, and opioid receptor involvement in an animal model of binge drinking

Michelle A. Tanchuck, Naomi Yoneyama, Matthew Ford, Andrea M. Fretwell, Deborah (Deb) Finn

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Drinking to intoxication or binge drinking is a hallmark characteristic of alcohol abuse. Although hard to model in rodents, the scheduled high alcohol consumption (SHAC) procedure generates high, stable ethanol intake and blood ethanol concentrations in mice to levels consistent with definitions of binge drinking. The purpose of the present studies was to determine the effects of pharmacological manipulation of the opioidergic, glutamatergic, and γ-aminobutyric acid (GABA)ergic systems on binge drinking with the SHAC procedure. Parallel manipulations were conducted in mice trained in operant self-administration of either sucrose or ethanol. For the SHAC procedure, genetically heterogeneous Withdrawal Seizure Control mice were given varying periods of fluid access, with a 30-min ethanol session every third day (total of seven). Mice were pretreated intraperitoneally with naltrexone (0, 0.6, or 1.25. mg/kg), baclofen (0, 2.5, or 5.0. mg/kg), or 2-methyl-6-(phenylethynyl)-pyridine (MPEP; 0, 3.0, or 10.0. mg/kg) before each ethanol session. For the operant self-administration procedure, separate groups of C57BL/6 mice were trained to complete a single response requirement (16 presses on the active lever) to gain 30. min of access to an ethanol or a sucrose solution. Mice received pretreatments of the same doses of naltrexone, MPEP, or baclofen before the self-administration sessions, with saline injections on intervening days. Naltrexone produced a dose-dependent decrease in binge drinking, and the highest dose also significantly decreased operant self-administration of ethanol and sucrose. Both doses of baclofen significantly decreased binge alcohol consumption, but the higher dose also tended to decrease water intake. The highest dose of baclofen also significantly decreased operant self-administration of sucrose. MPEP (10. mg/kg) significantly decreased binge alcohol consumption and sucrose self-administration. These results indicate that manipulation of the opioidergic, glutamatergic, and GABAergic systems significantly decreased binge drinking.

Original languageEnglish (US)
Pages (from-to)33-44
Number of pages12
JournalAlcohol
Volume45
Issue number1
DOIs
StatePublished - Feb 2011

Fingerprint

Aminobutyrates
Binge Drinking
self-administration
Metabotropic Glutamate Receptors
Opioid Receptors
Self Administration
alcohol consumption
Animals
Ethanol
Animal Models
animal
Baclofen
Alcohol Drinking
Alcohols
Sucrose
Naltrexone
manipulation
Drinking
seizure
intoxication

Keywords

  • Alcohol
  • Baclofen
  • Mice
  • MPEP
  • Naltrexone
  • Operant

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neurology
  • Toxicology
  • Health(social science)

Cite this

Assessment of GABA-B, metabotropic glutamate, and opioid receptor involvement in an animal model of binge drinking. / Tanchuck, Michelle A.; Yoneyama, Naomi; Ford, Matthew; Fretwell, Andrea M.; Finn, Deborah (Deb).

In: Alcohol, Vol. 45, No. 1, 02.2011, p. 33-44.

Research output: Contribution to journalArticle

Tanchuck, Michelle A. ; Yoneyama, Naomi ; Ford, Matthew ; Fretwell, Andrea M. ; Finn, Deborah (Deb). / Assessment of GABA-B, metabotropic glutamate, and opioid receptor involvement in an animal model of binge drinking. In: Alcohol. 2011 ; Vol. 45, No. 1. pp. 33-44.
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