Assessing genetic-based therapies for AIDS using the simian immunodeficiency virus.

C. L. Endres, E. Bergquam, Michael Axthelm, Scott Wong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A plasmid encoding the full-length infectious molecular proviral clone of SIVmac239 was generated. Virus derived from cells transfected with this clone replicated to high levels and was cytopathic for some transformed human CD4+ cell lines and primary rhesus macaque peripheral blood mononuclear cells. Since replication of SIV requires the functional expression of the viral encoded rev protein, transient co-transfection studies were initiated with the infectious proviral clone and a well-characterized trans-dominant negative HIV-1 rev mutant.

Original languageEnglish (US)
Pages (from-to)141-144
Number of pages4
JournalJournal of Medical Primatology
Volume24
Issue number3
StatePublished - May 1995

Fingerprint

Simian immunodeficiency virus
Simian Immunodeficiency Virus
Genetic Therapy
Acquired Immunodeficiency Syndrome
Clone Cells
clones
therapeutics
rev Gene Products
transfection
mononuclear leukocytes
Human immunodeficiency virus 1
Macaca mulatta
Transfection
HIV-1
Blood Cells
plasmids
Plasmids
cell lines
Viruses
Cell Line

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Assessing genetic-based therapies for AIDS using the simian immunodeficiency virus. / Endres, C. L.; Bergquam, E.; Axthelm, Michael; Wong, Scott.

In: Journal of Medical Primatology, Vol. 24, No. 3, 05.1995, p. 141-144.

Research output: Contribution to journalArticle

@article{0a45d72231ab48f38fd3482e3aea46bd,
title = "Assessing genetic-based therapies for AIDS using the simian immunodeficiency virus.",
abstract = "A plasmid encoding the full-length infectious molecular proviral clone of SIVmac239 was generated. Virus derived from cells transfected with this clone replicated to high levels and was cytopathic for some transformed human CD4+ cell lines and primary rhesus macaque peripheral blood mononuclear cells. Since replication of SIV requires the functional expression of the viral encoded rev protein, transient co-transfection studies were initiated with the infectious proviral clone and a well-characterized trans-dominant negative HIV-1 rev mutant.",
author = "Endres, {C. L.} and E. Bergquam and Michael Axthelm and Scott Wong",
year = "1995",
month = "5",
language = "English (US)",
volume = "24",
pages = "141--144",
journal = "Journal of Medical Primatology",
issn = "0047-2565",
publisher = "Blackwell Munksgaard",
number = "3",

}

TY - JOUR

T1 - Assessing genetic-based therapies for AIDS using the simian immunodeficiency virus.

AU - Endres, C. L.

AU - Bergquam, E.

AU - Axthelm, Michael

AU - Wong, Scott

PY - 1995/5

Y1 - 1995/5

N2 - A plasmid encoding the full-length infectious molecular proviral clone of SIVmac239 was generated. Virus derived from cells transfected with this clone replicated to high levels and was cytopathic for some transformed human CD4+ cell lines and primary rhesus macaque peripheral blood mononuclear cells. Since replication of SIV requires the functional expression of the viral encoded rev protein, transient co-transfection studies were initiated with the infectious proviral clone and a well-characterized trans-dominant negative HIV-1 rev mutant.

AB - A plasmid encoding the full-length infectious molecular proviral clone of SIVmac239 was generated. Virus derived from cells transfected with this clone replicated to high levels and was cytopathic for some transformed human CD4+ cell lines and primary rhesus macaque peripheral blood mononuclear cells. Since replication of SIV requires the functional expression of the viral encoded rev protein, transient co-transfection studies were initiated with the infectious proviral clone and a well-characterized trans-dominant negative HIV-1 rev mutant.

UR - http://www.scopus.com/inward/record.url?scp=0029296220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029296220&partnerID=8YFLogxK

M3 - Article

C2 - 8751053

AN - SCOPUS:0029296220

VL - 24

SP - 141

EP - 144

JO - Journal of Medical Primatology

JF - Journal of Medical Primatology

SN - 0047-2565

IS - 3

ER -