TY - JOUR
T1 - Assessing confocal microscopy systems for purchase
AU - Steyger, Peter
N1 - Funding Information:
The author acknowledges the contributions of Steve Pfieffer (No-ran), Brendan Yee (Bio-Rad), and, in particular, manuscript discussions with Dennis Trune, Ph.D., OHRC. This work has been supported by NIDCD 03028, Oregon Hearing Research Center, and capital equipment funds from the Murdock Foundation, Washington.
PY - 1999/8
Y1 - 1999/8
N2 - Confocal laser scanning microscopy now has so many functions and applications that choosing a new multiuser confocal laser microscopy system can be a daunting task, particularly for a first-time buyer and new users of confocal microscopy. How does one determine which features are most appropriate for any particular laboratory, application, or imaging environment? Each confocal microscopy system has its own individual advantages and limitations, which ultimately defines its market niche. Here, we describe the features that differentiate the four confocal microscopy systems we assessed. The decisive factors in choosing a confocal microscopy system for our anatomical laboratory were user-friendly software for on-line acquisition and off-line processing; the working distances of objective lenses; applicability for a multiuser environment; interactions with company representatives; and turnaround times for questions, service, and accessories.
AB - Confocal laser scanning microscopy now has so many functions and applications that choosing a new multiuser confocal laser microscopy system can be a daunting task, particularly for a first-time buyer and new users of confocal microscopy. How does one determine which features are most appropriate for any particular laboratory, application, or imaging environment? Each confocal microscopy system has its own individual advantages and limitations, which ultimately defines its market niche. Here, we describe the features that differentiate the four confocal microscopy systems we assessed. The decisive factors in choosing a confocal microscopy system for our anatomical laboratory were user-friendly software for on-line acquisition and off-line processing; the working distances of objective lenses; applicability for a multiuser environment; interactions with company representatives; and turnaround times for questions, service, and accessories.
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U2 - 10.1006/meth.1999.0811
DO - 10.1006/meth.1999.0811
M3 - Article
C2 - 10491273
AN - SCOPUS:0032728009
SN - 1046-2023
VL - 18
SP - 435
EP - 446
JO - Methods: A Companion to Methods in Enzymology
JF - Methods: A Companion to Methods in Enzymology
IS - 4
ER -