Assembly of HIV GAG-B-Galactosidase Fusion Proteins into Virus Particles

Chin Tien Wang, Jenny Stegeman-Olsen, Yaqiang Zhang, Eric Barklis

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We have studied the assembly of human immunodeficiency virus (HIV-1) Gag-B-galactosidase (Gag-B-gal; GBG) fusion proteins into HIV particles in the presence of HIV Gag proteins. Release of fusion proteins from cells was measured by assay of media versus cellular B-gat activities and was dependent on co-expression of unfused Gag proteins. Gag-B-gal incorporation into virus particles was demonstrated by detergent treatment and density gradient fractionation studies and was dependent on protein-protein interactions requiring the C-terminal two-thirds of the HIV CA domain. The central MA domain appeared unimportant for fusion protein incorporation; a nonmyristylated GBG protein was incorporated but at a relatively reduced level, while the NC and p6 domains slightly affected the assembly of fusion proteins into particles. Subcellular fractionation studies showed that all fusion proteins including the nonmyristylated one were enriched in the cytoplasmic pellet fraction. However, assembly into particles did not correlate with subcellular fractionstions patterns. Similarly, virion incorporation levels of Gag-B-gel proteins did not correlate with their immunofluorescence localization patterns. However, we observed that while most fusion proteins displayed a perinuclear ring with heterogeneous staining throughout cells, short fusion proteins appeared enriched on the intracellular membranes, and fusion proteins with intact MA but deleted NC domains showed an enhanced surface staining without a clear perinuclear ring. Altogether, our data suggest that the CA domain is the primary determinant for assembly of HIV fusion proteins into virus particles.

Original languageEnglish (US)
Pages (from-to)524-534
Number of pages11
JournalVirology
Volume200
Issue number2
DOIs
StatePublished - May 1 1994

Fingerprint

Galactosidases
Virion
HIV
Human Immunodeficiency Virus Proteins
Proteins
gag Gene Products
Cell Fusion
HIV-1
Membrane Fusion Proteins
Staining and Labeling
Intracellular Membranes
Detergents
Fluorescent Antibody Technique
Gels

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Assembly of HIV GAG-B-Galactosidase Fusion Proteins into Virus Particles. / Wang, Chin Tien; Stegeman-Olsen, Jenny; Zhang, Yaqiang; Barklis, Eric.

In: Virology, Vol. 200, No. 2, 01.05.1994, p. 524-534.

Research output: Contribution to journalArticle

Wang, Chin Tien ; Stegeman-Olsen, Jenny ; Zhang, Yaqiang ; Barklis, Eric. / Assembly of HIV GAG-B-Galactosidase Fusion Proteins into Virus Particles. In: Virology. 1994 ; Vol. 200, No. 2. pp. 524-534.
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