Assembly of epithelial cell fibrillins

Bette J. Dzamba, Douglas R. Keene, Zenzo Isogai, Noe L. Charbonneau, Nevena Karaman-Jurukovska, Marcia Simon, Lynn Sakai

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Fibrillins are large structural macromolecules that are components of connective tissue microfibrils. Fibrillin microfibrils have been found in association with basement membranes, where microfibrils appear to insert directly into the lamina densa. It is unknown whether fibrillins are limited to these sites of microfibril insertion or are present throughout the lamina densa. In this study, electron microscopic immunolocalization demonstrated the presence of fibrillin-1 throughout the lamina densa in the dermal- epidermal junction. In order to investigate whether fibrillin microfibrils might be present in the lamina densa, epithelial cell cultures (WISH, HaCaT, and primary keratinocytes) were analyzed by immunofluorescence, immunoblotting, and extraction of microfibrils followed by rotary shadowing electron microscopy and compared to mesenchymal cell cultures (dermal fibroblasts and MG63 osteosarcoma). In contrast to mesenchymal cells, which elaborate a fibrillin fibril network, epithelial cells primarily deposit fibrillin into the extracellular matrix in a non-fibrillar form. Coculture experiments using human epithelial cells and mouse fibroblasts implicated the cells themselves in the assembly of fibrillin. The importance of the cell in this process was further underscored by novel data demonstrating that keratinocytes selectively secrete fibrillin-1 into the matrix and not into the medium and can differentiate between fibrillin-1 and fibrillin-2.

Original languageEnglish (US)
Pages (from-to)1612-1620
Number of pages9
JournalJournal of Investigative Dermatology
Volume117
Issue number6
DOIs
StatePublished - Jan 1 2001

Fingerprint

Microfibrils
Epithelial Cells
Fibroblasts
Cell culture
Macromolecules
Electron microscopy
Keratinocytes
Deposits
Tissue
Cell Culture Techniques
Electrons
Skin
Osteosarcoma
Coculture Techniques
Fibrillins
Immunoblotting
Basement Membrane
Connective Tissue
Fluorescent Antibody Technique
Extracellular Matrix

Keywords

  • Basement membranes
  • Co-cultures
  • Fibrillin
  • Marfan gene
  • Micro-fibrils

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Dzamba, B. J., Keene, D. R., Isogai, Z., Charbonneau, N. L., Karaman-Jurukovska, N., Simon, M., & Sakai, L. (2001). Assembly of epithelial cell fibrillins. Journal of Investigative Dermatology, 117(6), 1612-1620. https://doi.org/10.1046/j.0022-202x.2001.01588.x

Assembly of epithelial cell fibrillins. / Dzamba, Bette J.; Keene, Douglas R.; Isogai, Zenzo; Charbonneau, Noe L.; Karaman-Jurukovska, Nevena; Simon, Marcia; Sakai, Lynn.

In: Journal of Investigative Dermatology, Vol. 117, No. 6, 01.01.2001, p. 1612-1620.

Research output: Contribution to journalArticle

Dzamba, BJ, Keene, DR, Isogai, Z, Charbonneau, NL, Karaman-Jurukovska, N, Simon, M & Sakai, L 2001, 'Assembly of epithelial cell fibrillins', Journal of Investigative Dermatology, vol. 117, no. 6, pp. 1612-1620. https://doi.org/10.1046/j.0022-202x.2001.01588.x
Dzamba BJ, Keene DR, Isogai Z, Charbonneau NL, Karaman-Jurukovska N, Simon M et al. Assembly of epithelial cell fibrillins. Journal of Investigative Dermatology. 2001 Jan 1;117(6):1612-1620. https://doi.org/10.1046/j.0022-202x.2001.01588.x
Dzamba, Bette J. ; Keene, Douglas R. ; Isogai, Zenzo ; Charbonneau, Noe L. ; Karaman-Jurukovska, Nevena ; Simon, Marcia ; Sakai, Lynn. / Assembly of epithelial cell fibrillins. In: Journal of Investigative Dermatology. 2001 ; Vol. 117, No. 6. pp. 1612-1620.
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