Assembly and specific recognition of K29- and K33-linked polyubiquitin

Martin A. Michel, Paul R. Elliott, Kirby N. Swatek, Michal Simicek, Jonathan Pruneda, Jane L. Wagstaff, Stefan M.V. Freund, David Komander

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Protein ubiquitination regulates many cellular processes via attachment of structurally and functionally distinct ubiquitin (Ub) chains. Several atypical chain types have remained poorly characterized because the enzymes mediating their assembly and receptors with specific binding properties have been elusive. We found that the human HECT E3 ligases UBE3C and AREL1 assemble K48/K29- and K11/K33-linked Ub chains, respectively, and can be used in combination with DUBs to generate K29- and K33-linked chains for biochemical and structural analyses. Solution studies indicate that both chains adopt open anddynamic conformations. We further show that the N-terminal Npl4-like zinc finger (NZF1) domain of the K29/K33-specific deubiquitinase TRABID specifically binds K29/K33-linked diUb, and a crystal structure of this complex explains TRABID specificity and suggests a model for chain binding by TRABID. Our work uncovers linkage-specific components in the Ub system for atypical K29- and K33-linked Ub chains, providing tools to further understand these unstudied posttranslational modifications.

Original languageEnglish (US)
Pages (from-to)95-109
Number of pages15
JournalMolecular Cell
Volume58
Issue number1
DOIs
StatePublished - Apr 2 2015
Externally publishedYes

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Polyubiquitin
Ubiquitin
Ubiquitin-Protein Ligases
Ubiquitination
Zinc Fingers
Post Translational Protein Processing
Enzymes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Michel, M. A., Elliott, P. R., Swatek, K. N., Simicek, M., Pruneda, J., Wagstaff, J. L., ... Komander, D. (2015). Assembly and specific recognition of K29- and K33-linked polyubiquitin. Molecular Cell, 58(1), 95-109. https://doi.org/10.1016/j.molcel.2015.01.042

Assembly and specific recognition of K29- and K33-linked polyubiquitin. / Michel, Martin A.; Elliott, Paul R.; Swatek, Kirby N.; Simicek, Michal; Pruneda, Jonathan; Wagstaff, Jane L.; Freund, Stefan M.V.; Komander, David.

In: Molecular Cell, Vol. 58, No. 1, 02.04.2015, p. 95-109.

Research output: Contribution to journalArticle

Michel, MA, Elliott, PR, Swatek, KN, Simicek, M, Pruneda, J, Wagstaff, JL, Freund, SMV & Komander, D 2015, 'Assembly and specific recognition of K29- and K33-linked polyubiquitin', Molecular Cell, vol. 58, no. 1, pp. 95-109. https://doi.org/10.1016/j.molcel.2015.01.042
Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda J, Wagstaff JL et al. Assembly and specific recognition of K29- and K33-linked polyubiquitin. Molecular Cell. 2015 Apr 2;58(1):95-109. https://doi.org/10.1016/j.molcel.2015.01.042
Michel, Martin A. ; Elliott, Paul R. ; Swatek, Kirby N. ; Simicek, Michal ; Pruneda, Jonathan ; Wagstaff, Jane L. ; Freund, Stefan M.V. ; Komander, David. / Assembly and specific recognition of K29- and K33-linked polyubiquitin. In: Molecular Cell. 2015 ; Vol. 58, No. 1. pp. 95-109.
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