Assembly and characterization of biofunctional neurotransmitter-immobilized surfaces for interaction with postsynaptic membrane receptors

Uroosa Saifuddin, Tothu (Tania) Vu, Miroslav Rezac, Haohua Qian, David R. Pepperberg, Tejal A. Desai

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Herein, we report progress toward the development of bioactive surfaces based on γ-aminobutyric acid (GABA), a major neurotransmitter in the nervous system. Whereas immobilization techniques have focused largely on antibodies, enzymes, and receptors, to our knowledge, this is the first report of a prototype neurotransmitter-immobilized surface. Biosurfaces were assembled onto either mica or glass using passive adsorption of avidin and subsequent attachment of a derivatized form of GABA via a biotinavidin affinity bond. Surface characterization of these prepared bimolecular surfaces was determined using atomic force microscopy in tapping mode. The data reveal that passive adsorption of avidin is uniformly dispersed and cluster densities can be controlled through the concentration of the avidin incubation solution. GABA tethered via biotin to these avidin surfaces displayed a unique surface topology; in addition, histograms of surface heights suggest two different types of molecular cluster populations. Functional assays were performed to test the biological activity of the synthesized GABA. Anti-GABA antibody directed to these bimolecular surfaces result in morphological topologies and histograms that indicate antibody-antigen binding. However, nonspecific anti-immunoglobulin G antibodies directed to these surfaces show low binding affinity. Taken together, the data support the idea that the synthesized surfaces are biofunctional.

Original languageEnglish (US)
Pages (from-to)184-191
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume66
Issue number1
StatePublished - Jul 1 2003
Externally publishedYes

Fingerprint

Neurotransmitter Agents
Membranes
Avidin
gamma-Aminobutyric Acid
Antibodies
Topology
Aminobutyrates
Adsorption
Mica
Neurology
Antigens
Biotin
Bioactivity
Assays
Atomic force microscopy
Enzymes
Immunoglobulin G
Glass
Acids

Keywords

  • AFM
  • Biosurface
  • Immobilization
  • Neurotransmitter
  • Solid substrate

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Assembly and characterization of biofunctional neurotransmitter-immobilized surfaces for interaction with postsynaptic membrane receptors. / Saifuddin, Uroosa; Vu, Tothu (Tania); Rezac, Miroslav; Qian, Haohua; Pepperberg, David R.; Desai, Tejal A.

In: Journal of Biomedical Materials Research - Part A, Vol. 66, No. 1, 01.07.2003, p. 184-191.

Research output: Contribution to journalArticle

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