Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin

Bin Gao, Raju Adhikari, Mark Howarth, Kimitoshi Nakamura, Marielle Gold, Ann Hill, Rai Knee, Marek Michalak, Tim Elliott

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

MHC class I molecules expressed in a calreticulin-deficient cell line (K42) assembled with β 2-microglobulin (β2-m) normally, but their subsequent loading with optimal peptides was defective. Suboptimally loaded class I molecules were released into the secretory pathway. This occurred despite the ability of newly synthesized class I to interact with the transporter associated with antigen processing (TAP) loading complex. The efficiency of peptide loading was reduced by 50%-80%, and impaired T cell recognition was observed for three out of four antigens tested. The peptide-loading function was specific to calreticulin, since the defect in K42 could be rectified by transfection with calreticulin but not a soluble form of calnexin, which shares its lectin-like activity.

Original languageEnglish (US)
Pages (from-to)99-109
Number of pages11
JournalImmunity
Volume16
Issue number1
DOIs
StatePublished - 2002

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Calreticulin
Antigens
Peptides
Calnexin
Secretory Pathway
Lectins
Transfection
T-Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin. / Gao, Bin; Adhikari, Raju; Howarth, Mark; Nakamura, Kimitoshi; Gold, Marielle; Hill, Ann; Knee, Rai; Michalak, Marek; Elliott, Tim.

In: Immunity, Vol. 16, No. 1, 2002, p. 99-109.

Research output: Contribution to journalArticle

Gao, B, Adhikari, R, Howarth, M, Nakamura, K, Gold, M, Hill, A, Knee, R, Michalak, M & Elliott, T 2002, 'Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin', Immunity, vol. 16, no. 1, pp. 99-109. https://doi.org/10.1016/S1074-7613(01)00260-6
Gao, Bin ; Adhikari, Raju ; Howarth, Mark ; Nakamura, Kimitoshi ; Gold, Marielle ; Hill, Ann ; Knee, Rai ; Michalak, Marek ; Elliott, Tim. / Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin. In: Immunity. 2002 ; Vol. 16, No. 1. pp. 99-109.
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AU - Gold, Marielle

AU - Hill, Ann

AU - Knee, Rai

AU - Michalak, Marek

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