Aspartate 19 and glutamate 121 are critical for transport function of the myo-inositol/H+ symporter from Leishmania donovani

Andreas Seyfang, Michael P. Kavanaugh, Scott Landfear

Research output: Contribution to journalArticle

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Abstract

The protozoan flagellate Leishmania donovani has an active myo- inositol/proton symporter (MIT), which is driven by a proton gradient across the parasite membrane. We have used site-directed mutagenesis in combination with functional expression of transporter mutants in Xenopus oocytes and overexpression in Leishmania transfectants to investigate the significance of acidic transmembrane residues for proton relay and inositol transport. MIT has only three charged amino acids within predicted transmembrane domains. Two of these residues, Asp19 (TM1) and Glu121 (TM4), appeared to be critical for transport function of MIT, with a reduction of inositol transport to about 2% of wild-type activity when mutated to the uncharged amides D19N or E121Q and 20% (D19E) or 4% (E121D) of wild-type activity for the conservative mutations that retained the charge. Immunofluorescence microscopy of oocyte cryosections showed that MIT mutants were expressed on the oocyte surface at a similar level as MIT wild type, confirming that these mutations affect transport function and do not prevent trafficking of the transporter to the plasma membrane. The proton uncouplers carbonylcyanide-4- (trifluoromethoxy)phenylhydrazone and dinitrophenol inhibited inositol transport by 50-70% in the wild-type as well as in E121Q, despite its reduced transport activity. The mutant D19N, however, was stimulated about 4-fold by either protonophore and 2-fold by cyanide or increase of pH 7.5 to 8.5 but inhibited at pH 6.5. The conservative mutant D19E, in contrast, showed an inhibition profile similar to MIT wild type. We conclude that Asp19 and Glu121 are critical for myo-inositol transport, while the negatively charged carboxylate at Asp19 may be important for proton coupling of MIT.

Original languageEnglish (US)
Pages (from-to)24210-24215
Number of pages6
JournalJournal of Biological Chemistry
Volume272
Issue number39
DOIs
StatePublished - Sep 26 1997

Fingerprint

Symporters
Leishmania donovani
Inositol
Aspartic Acid
Protons
Glutamic Acid
Oocytes
Dinitrophenols
Mutagenesis
Mutation
Leishmania
Cyanides
Cell membranes
Xenopus
Site-Directed Mutagenesis
Fluorescence Microscopy
Amides
Microscopic examination
Parasites

ASJC Scopus subject areas

  • Biochemistry

Cite this

Aspartate 19 and glutamate 121 are critical for transport function of the myo-inositol/H+ symporter from Leishmania donovani. / Seyfang, Andreas; Kavanaugh, Michael P.; Landfear, Scott.

In: Journal of Biological Chemistry, Vol. 272, No. 39, 26.09.1997, p. 24210-24215.

Research output: Contribution to journalArticle

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abstract = "The protozoan flagellate Leishmania donovani has an active myo- inositol/proton symporter (MIT), which is driven by a proton gradient across the parasite membrane. We have used site-directed mutagenesis in combination with functional expression of transporter mutants in Xenopus oocytes and overexpression in Leishmania transfectants to investigate the significance of acidic transmembrane residues for proton relay and inositol transport. MIT has only three charged amino acids within predicted transmembrane domains. Two of these residues, Asp19 (TM1) and Glu121 (TM4), appeared to be critical for transport function of MIT, with a reduction of inositol transport to about 2{\%} of wild-type activity when mutated to the uncharged amides D19N or E121Q and 20{\%} (D19E) or 4{\%} (E121D) of wild-type activity for the conservative mutations that retained the charge. Immunofluorescence microscopy of oocyte cryosections showed that MIT mutants were expressed on the oocyte surface at a similar level as MIT wild type, confirming that these mutations affect transport function and do not prevent trafficking of the transporter to the plasma membrane. The proton uncouplers carbonylcyanide-4- (trifluoromethoxy)phenylhydrazone and dinitrophenol inhibited inositol transport by 50-70{\%} in the wild-type as well as in E121Q, despite its reduced transport activity. The mutant D19N, however, was stimulated about 4-fold by either protonophore and 2-fold by cyanide or increase of pH 7.5 to 8.5 but inhibited at pH 6.5. The conservative mutant D19E, in contrast, showed an inhibition profile similar to MIT wild type. We conclude that Asp19 and Glu121 are critical for myo-inositol transport, while the negatively charged carboxylate at Asp19 may be important for proton coupling of MIT.",
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