Abstract
Chemotactic factors can cause polymorphonuclear leukocytes to release the contents of azurophilic granules, including the enzymes β-glucuronidase and myeloperoxidase. In the presence of aqueous humor from the anterior chamber of the rabbit eye, the supernatant from stimulated leukocytes contains β-glucuronidase, but myeloperoxidase is not detectable. Studies with aqueous humor and partially purified human myeloperoxidase suggest that this phenomenon is not due to a failure of enzyme release. The factor responsible for the inability to detect MPO in the assay system is heat-labile, dialyzable, and reversed by ascorbate oxidase. Comparable assay inhibition is produced by ascorbic acid at a concentration present in either human or rabbit aqueous humor. The ability of aqueous humor to protect against myeloperoxidase-induced oxidation may contribute to several diverse phenomena, including the susceptibility of the eye to Candida infection and a prolonged half-life for several inflammatory mediators in the anterior chamber.
Original language | English (US) |
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Pages (from-to) | 244-247 |
Number of pages | 4 |
Journal | American Journal of Pathology |
Volume | 120 |
Issue number | 2 |
State | Published - 1985 |
Externally published | Yes |
ASJC Scopus subject areas
- Pathology and Forensic Medicine