Ascorbate in aqueous humor protects against myeloperoxidase-induced oxidation

J. T. Rosenbaum, E. L. Howes, D. English

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Chemotactic factors can cause polymorphonuclear leukocytes to release the contents of azurophilic granules, including the enzymes β-glucuronidase and myeloperoxidase. In the presence of aqueous humor from the anterior chamber of the rabbit eye, the supernatant from stimulated leukocytes contains β-glucuronidase, but myeloperoxidase is not detectable. Studies with aqueous humor and partially purified human myeloperoxidase suggest that this phenomenon is not due to a failure of enzyme release. The factor responsible for the inability to detect MPO in the assay system is heat-labile, dialyzable, and reversed by ascorbate oxidase. Comparable assay inhibition is produced by ascorbic acid at a concentration present in either human or rabbit aqueous humor. The ability of aqueous humor to protect against myeloperoxidase-induced oxidation may contribute to several diverse phenomena, including the susceptibility of the eye to Candida infection and a prolonged half-life for several inflammatory mediators in the anterior chamber.

Original languageEnglish (US)
Pages (from-to)244-247
Number of pages4
JournalAmerican Journal of Pathology
Volume120
Issue number2
StatePublished - Dec 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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