Ascorbate-enhanced chondrogenesis of ATDC5 cells

F. M. Altaf, T. M. Hering, N. H. Kazmi, Jung Yoo, Brian Johnstone

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The ATDC5 cell line exhibits the multistep chondrogenic differentiation observed during endochondral bone formation. However, it takes up to two months to complete the process of cell expansion, insulin addition to promote differentiation and further changes in culture conditions effectively to induce hypertrophy. We sought to produce consistent chondrogenesis with significant hypertrophic differentiation with simpler conditions in a more practical time period. By adding ascorbate, the prechondrogenic proliferation phase was shortened from 21 to 7 days, with production of cartilaginous nodules during the chondrogenic phase, after insulin addition, that were greater in number and larger in size. Immunohistochemistry indicated much greater matrix elaboration and the mRNA expression of sox9, aggrecan and collagen type II were all significantly increased earlier and to a much higher degree when compared with controls. Moreover, there was a robust induction of hypertrophy: Col10a1, Runx2 and Mmp13 were all induced within 7-10 days. In conclusion, addition of ascorbate to ATDC5 cultures shortened the prechondrogenic proliferation phase, produced earlier chondrogenic differentiation, heightened gene expression and robust hypertrophic differentiation, abrogating the need for extended culture times and the changes in culture conditions. This simple modification considerably enhances the practicality of this cell line for studies of chondrogenesis.

Original languageEnglish (US)
Pages (from-to)64-69
Number of pages6
JournalEuropean Cells and Materials
Volume12
StatePublished - Jul 2006

Fingerprint

Chondrogenesis
Insulin
Hypertrophy
Cells
Aggrecans
Cell Line
Collagen Type II
Collagen
Osteogenesis
Gene expression
Bone
Immunohistochemistry
Gene Expression
Messenger RNA

Keywords

  • Ascorbate
  • ATDC5 cells
  • Chondrogenesis
  • Differentiation
  • Hypertrophy

ASJC Scopus subject areas

  • Biochemistry
  • Biomaterials

Cite this

Ascorbate-enhanced chondrogenesis of ATDC5 cells. / Altaf, F. M.; Hering, T. M.; Kazmi, N. H.; Yoo, Jung; Johnstone, Brian.

In: European Cells and Materials, Vol. 12, 07.2006, p. 64-69.

Research output: Contribution to journalArticle

Altaf, F. M. ; Hering, T. M. ; Kazmi, N. H. ; Yoo, Jung ; Johnstone, Brian. / Ascorbate-enhanced chondrogenesis of ATDC5 cells. In: European Cells and Materials. 2006 ; Vol. 12. pp. 64-69.
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