TY - JOUR
T1 - ARID1A and CEBPα cooperatively inhibit UCA1 transcription in breast cancer
AU - Guo, Xiao
AU - Zhang, Yin
AU - Mayakonda, Anand
AU - Madan, Vikas
AU - Ding, Ling Wen
AU - Lin, Le Hang
AU - Zia, Saadiya
AU - Gery, Sigal
AU - Tyner, Jeffrey W.
AU - Zhou, Wu
AU - Yin, Dong
AU - Lin, De Chen
AU - Koeffler, H. Phillip
N1 - Publisher Copyright:
© 2018, Macmillan Publishers Limited, part of Springer Nature.
PY - 2018/11/8
Y1 - 2018/11/8
N2 - As one of the primary members of SWI/SNF chromatin remodeling complexes, ARID1A contains frequent loss-of-function mutations in many types of cancers. However, the molecular mechanisms underlying ARID1A deficiency in cancer biology remain to be investigated. Using breast cancer as a model, we report that silencing ARID1A significantly increased cellular proliferation and migration. Mechanistically, primarily functioning as a transcriptional repressor, loss of ARID1A profoundly alters histone modifications and the transcriptome. Notably, ARID1A inhibited the expression of a long non-coding RNA, UCA1, by regulating chromatin access of the transcription factor CEBPα. Restoration experiments showed that UCA1 mediates the functions of ARID1A that induces loss of cellular proliferation and migration. Together, our findings characterize ARID1A as a key tumor-suppressor gene in breast cancer through cooperation with CEBPα, and loss-of-function mutations of ARID1A activates UCA1.
AB - As one of the primary members of SWI/SNF chromatin remodeling complexes, ARID1A contains frequent loss-of-function mutations in many types of cancers. However, the molecular mechanisms underlying ARID1A deficiency in cancer biology remain to be investigated. Using breast cancer as a model, we report that silencing ARID1A significantly increased cellular proliferation and migration. Mechanistically, primarily functioning as a transcriptional repressor, loss of ARID1A profoundly alters histone modifications and the transcriptome. Notably, ARID1A inhibited the expression of a long non-coding RNA, UCA1, by regulating chromatin access of the transcription factor CEBPα. Restoration experiments showed that UCA1 mediates the functions of ARID1A that induces loss of cellular proliferation and migration. Together, our findings characterize ARID1A as a key tumor-suppressor gene in breast cancer through cooperation with CEBPα, and loss-of-function mutations of ARID1A activates UCA1.
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U2 - 10.1038/s41388-018-0371-4
DO - 10.1038/s41388-018-0371-4
M3 - Article
C2 - 29980791
AN - SCOPUS:85049598128
SN - 0950-9232
VL - 37
SP - 5939
EP - 5951
JO - Oncogene
JF - Oncogene
IS - 45
ER -