Are proteasomes involved in the formation of the kinetochore?

N. Paweletz, C. Wójcik, D. Schroeter, E. M. Finze

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Proteasomes catalyse the degradation of proteins responsible for the regulation of mitosis enabling the cell to complete cell division. We have studied the effect of an inhibitor of the chymotrypsin-like activity of the proteasome on the trilaminar structure of the kinetochore in HeLa cells. Whereas a role for the proteasome in the degeneration of the kinetochore was predicted, we found instead that the inhibitor strongly retarded kinetochore development. We observed different 'developmental' stages of the kinetochore from the fibrous ball of a 'prekinetochore' to the 'mature' kinetochore in one cell. The data presented here support the proposition that proteasomes are involved in kinetochore formation.

Original languageEnglish (US)
Pages (from-to)436-442
Number of pages7
JournalChromosome Research
Volume4
Issue number6
DOIs
StatePublished - Jan 1 1996

Keywords

  • Cell cycle regulation
  • Centromere
  • Kinetochore
  • Microtubules
  • Mitosis
  • Proteasomes

ASJC Scopus subject areas

  • Genetics

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