Are human luteinizing granulosa cells a site of action for progesterone and relaxin?

L. H. Greenberg, Richard Stouffer, R. M. Brenner, T. A. Molskness, S. A. Hild-Petito, Q. Yu

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Specific nuclear staining for progesterone receptor (PR) was detected by immunocytochemistry in human granulosa cells (GCs) obtained from in vitro fertilization protocols. The percent of PR-positive cells (60% to 80%) remained unchanged during 7 days of culture in media containing fetal calf serum, in the absence of human chorionic gonadotropin (hCG) or the progesterone antagonist RU486. Progesterone (P) production by GCs cultured on extracellular matrix from bovine corneal endothelial cells was stimulated by hCG and prostaglandin E2 (PGE2). However, addition of RU486 or human relaxin had no effect on control, hCG-, or PGE2-stimulated P production. Thus, the receptor data are consistent with an autocrine action of P in luteinizing GCs, but initial experiments in cell culture did not define a role for P or relaxin in modulating luteal steroidogenesis.

    Original languageEnglish (US)
    Pages (from-to)446-453
    Number of pages8
    JournalFertility and Sterility
    Volume53
    Issue number3
    StatePublished - 1990

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    Relaxin
    Granulosa Cells
    Chorionic Gonadotropin
    Progesterone
    Progesterone Receptors
    Dinoprostone
    Corpus Luteum
    Fertilization in Vitro
    Extracellular Matrix
    Culture Media
    Endothelial Cells
    Cell Culture Techniques
    Immunohistochemistry
    Staining and Labeling
    Serum

    ASJC Scopus subject areas

    • Obstetrics and Gynecology

    Cite this

    Greenberg, L. H., Stouffer, R., Brenner, R. M., Molskness, T. A., Hild-Petito, S. A., & Yu, Q. (1990). Are human luteinizing granulosa cells a site of action for progesterone and relaxin? Fertility and Sterility, 53(3), 446-453.

    Are human luteinizing granulosa cells a site of action for progesterone and relaxin? / Greenberg, L. H.; Stouffer, Richard; Brenner, R. M.; Molskness, T. A.; Hild-Petito, S. A.; Yu, Q.

    In: Fertility and Sterility, Vol. 53, No. 3, 1990, p. 446-453.

    Research output: Contribution to journalArticle

    Greenberg, LH, Stouffer, R, Brenner, RM, Molskness, TA, Hild-Petito, SA & Yu, Q 1990, 'Are human luteinizing granulosa cells a site of action for progesterone and relaxin?', Fertility and Sterility, vol. 53, no. 3, pp. 446-453.
    Greenberg LH, Stouffer R, Brenner RM, Molskness TA, Hild-Petito SA, Yu Q. Are human luteinizing granulosa cells a site of action for progesterone and relaxin? Fertility and Sterility. 1990;53(3):446-453.
    Greenberg, L. H. ; Stouffer, Richard ; Brenner, R. M. ; Molskness, T. A. ; Hild-Petito, S. A. ; Yu, Q. / Are human luteinizing granulosa cells a site of action for progesterone and relaxin?. In: Fertility and Sterility. 1990 ; Vol. 53, No. 3. pp. 446-453.
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