Aqueous red-emitting silicon nanoparticles for cellular imaging

Consequences of protecting against surface passivation by hydroxide and water for stable red emission

Sheng Kuei Chiu, Beth A. Manhat, William J I De Benedetti, Anna L. Brown, Katye Fichter, Tothu (Tania) Vu, Micah Eastman, Jun Jiao, Andrea M. Goforth

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Stable, aqueous, red-to-near infrared emission is critical for the use of silicon nanoparticles (Si NPs) in biological fluorescence assays, but such Si NPs have been difficult to attain. We report a synthesis and surface modification strategy that protects Si NPs and preserves red photoluminescence (PL) in water for more than 6 mo. The Si NPs were synthesized via high temperature reaction, liberated from an oxide matrix, and functionalized via hydrosilylation to yield hydrophobic particles. The hydrophobic Si NPs were phase transferred to water using the surfactant cetyltrimethylammonium bromide (CTAB) with retention of red PL. CTAB apparently serves a double role in providing stable, aqueous, red-emitting Si NPs by (i) forming a hydrophobic barrier between the Si NPs and water and (ii) providing aqueous colloidal stability via the polar head group. We demonstrate preservation of the aqueous red emission of these Si NPs in biological media and examine the effects of pH on emission color.

Original languageEnglish (US)
Pages (from-to)216-230
Number of pages15
JournalJournal of Materials Research
Volume28
Issue number2
DOIs
StatePublished - Jan 28 2013
Externally publishedYes

Fingerprint

Silicon
Passivation
passivity
hydroxides
Nanoparticles
Imaging techniques
nanoparticles
Water
silicon
water
bromides
Photoluminescence
photoluminescence
Hydrosilylation
hydroxide ion
Surface-Active Agents
Oxides
Surface treatment
Assays
Surface active agents

Keywords

  • aqueous colloid
  • cell uptake study
  • fluorescent imaging agent
  • PMT detector sensitivity
  • red fluorescence
  • semiconductor nanoparticle
  • silicon nanoparticle

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanical Engineering
  • Mechanics of Materials
  • Condensed Matter Physics

Cite this

Aqueous red-emitting silicon nanoparticles for cellular imaging : Consequences of protecting against surface passivation by hydroxide and water for stable red emission. / Chiu, Sheng Kuei; Manhat, Beth A.; De Benedetti, William J I; Brown, Anna L.; Fichter, Katye; Vu, Tothu (Tania); Eastman, Micah; Jiao, Jun; Goforth, Andrea M.

In: Journal of Materials Research, Vol. 28, No. 2, 28.01.2013, p. 216-230.

Research output: Contribution to journalArticle

Chiu, Sheng Kuei ; Manhat, Beth A. ; De Benedetti, William J I ; Brown, Anna L. ; Fichter, Katye ; Vu, Tothu (Tania) ; Eastman, Micah ; Jiao, Jun ; Goforth, Andrea M. / Aqueous red-emitting silicon nanoparticles for cellular imaging : Consequences of protecting against surface passivation by hydroxide and water for stable red emission. In: Journal of Materials Research. 2013 ; Vol. 28, No. 2. pp. 216-230.
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