Aqueous Oxygen Attenuation of Reperfusion Microvascular Ischemia in a Canine Model of Myocardial Infarction

J. Richard Spears, Petar Prcevski, Rui Xu, Li Li, Giles Brereton, Marcello Dicarli, Ali Spanta, Richard Crilly, Steven Lavine, Richard Vander Heide

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Uncorrected microvascular ischemia may contribute to left ventricular impairment during reperfusion after prolonged coronary artery occlusion. Attenuation of such ischemia in microvessels with impaired erythrocyte flow may require delivery of oxygen at high levels in plasma. Intraarterial infusion of aqueous oxygen (AO) can be used in a site specific manner to achieve hyperoxemic levels of oxygenation in the perfusate. With this new approach, the hypothesis was tested that reperfusion microvascular ischemia can be attenuated. After a 90 min coronary balloon occlusion in a canine model, AO hyperoxemic intracoronary perfusion was performed for 90 min after a 30 min period of autoreperfusion. Control groups consisted of normoxemic reperfusion, both passive (autoreperfusion) and active (roller pump). A significant improvement in left ventricular ejection fraction (p <0.05) at 2 hr of reperfusion was noted only in the AO hyperoxemia group (17 ± 6% by two dimensional echo), without a significant reduction in the improvement 1 hr after termination of treatment. During AO hyperoxemic perfusion, ECG ST segment isoelectric deviation normalized, and frequency of ventricular premature contractions was significantly reduced, in contrast to the autoreperfusion control group (p <0.05). Microvascular blood flow, measured as the ischemic/normal left ventricular segment ratio by radiolabeled microspheres immediately after AO hyperoxemic perfusion, was double the value of the autoreperfusion control group at 2 hr of reperfusion (p <0.05). We conclude that reperfusion microvascular ischemia is attenuated by intracoronary AO hyperoxemic perfusion and acutely improves left ventricular function in this model.

Original languageEnglish (US)
Pages (from-to)716-720
Number of pages5
JournalASAIO Journal
Volume49
Issue number6
DOIs
StatePublished - Nov 2003
Externally publishedYes

Fingerprint

Reperfusion
Canidae
Ischemia
Myocardial Infarction
Oxygen
Perfusion
Coronary Occlusion
Control Groups
Balloon Occlusion
Intra Arterial Infusions
Oxygenation
Ventricular Premature Complexes
Balloons
Microvessels
Electrocardiography
Microspheres
Left Ventricular Function
Stroke Volume
Coronary Vessels
Blood

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Spears, J. R., Prcevski, P., Xu, R., Li, L., Brereton, G., Dicarli, M., ... Vander Heide, R. (2003). Aqueous Oxygen Attenuation of Reperfusion Microvascular Ischemia in a Canine Model of Myocardial Infarction. ASAIO Journal, 49(6), 716-720. https://doi.org/10.1097/01.MAT.0000094665.72503.3C

Aqueous Oxygen Attenuation of Reperfusion Microvascular Ischemia in a Canine Model of Myocardial Infarction. / Spears, J. Richard; Prcevski, Petar; Xu, Rui; Li, Li; Brereton, Giles; Dicarli, Marcello; Spanta, Ali; Crilly, Richard; Lavine, Steven; Vander Heide, Richard.

In: ASAIO Journal, Vol. 49, No. 6, 11.2003, p. 716-720.

Research output: Contribution to journalArticle

Spears, JR, Prcevski, P, Xu, R, Li, L, Brereton, G, Dicarli, M, Spanta, A, Crilly, R, Lavine, S & Vander Heide, R 2003, 'Aqueous Oxygen Attenuation of Reperfusion Microvascular Ischemia in a Canine Model of Myocardial Infarction', ASAIO Journal, vol. 49, no. 6, pp. 716-720. https://doi.org/10.1097/01.MAT.0000094665.72503.3C
Spears, J. Richard ; Prcevski, Petar ; Xu, Rui ; Li, Li ; Brereton, Giles ; Dicarli, Marcello ; Spanta, Ali ; Crilly, Richard ; Lavine, Steven ; Vander Heide, Richard. / Aqueous Oxygen Attenuation of Reperfusion Microvascular Ischemia in a Canine Model of Myocardial Infarction. In: ASAIO Journal. 2003 ; Vol. 49, No. 6. pp. 716-720.
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