Aqueous humour concentrations of TGF-β, PLGF and FGF-1 and total retinal blood flow in patients with early non-proliferative diabetic retinopathy

Lee Anne Khuu, Faryan Tayyari, Jeremy M. Sivak, John G. Flanagan, Shaun Singer, Michael H. Brent, David Huang, Ou Tan, Christopher Hudson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: To correlate angiogenic cytokines in the aqueous humour with total retinal blood flow in subjects with type 2 diabetes with non-proliferative diabetic retinopathy (NPDR). Methods: A total of 17 controls and 16 NPDR patients were recruited into the study. Aqueous humour was collected at the start of cataract surgery to assess the concentration of 14 angiogenic cytokines. Aqueous humour was analysed using the suspension array method. Six images were acquired to assess total retinal blood flow (TRBF) using the prototype RTVue Doppler Fourier domain optical coherence tomography (Doppler FD-OCT) (Optovue, Inc., Fremont, CA) using a double circular scan protocol, 1 month postsurgery. At the same visit, forearm blood was collected to determine glycosylated haemoglobin (A1c). Results: Transforming growth factor beta (TGF-β1, TGF-β2) and PLGF were increased while FGF-1 was reduced in NPDR compared to controls (Bonferroni corrected, p < 0.003 for all). Total retinal blood flow (TRBF) was significantly reduced in the NPDR group compared to controls (33.1 ± 9.9 versus 43.3 ± 5.3 μl/min, p = 0.002). Aqueous FGF-1 significantly correlated with TRBF in the NPDR group (r = 0.71, p = 0.01; r2 = 0.51). In a multiple regression analysis, A1c was found to be a significant predictor of aqueous TGF-β1 and FGF-1 (p = 0.018 and p = 0.020, respectively). Conclusion: Aqueous angiogenic cytokines (TGF-β1, TGF-β2 and PLGF) were elevated in conjunction with a reduction in TRBF in patients with NPDR compared to controls. Non-invasive measurement of TRBF may be useful for predicting aqueous FGF-1 levels and severity of vasculopathy in DR.

Original languageEnglish (US)
JournalActa Ophthalmologica
DOIs
StateAccepted/In press - 2016

Fingerprint

Fibroblast Growth Factor 1
Aqueous Humor
Diabetic Retinopathy
Cytokines
Optical Coherence Tomography
Glycosylated Hemoglobin A
Forearm
Transforming Growth Factor beta
Cataract
Type 2 Diabetes Mellitus
Suspensions
Regression Analysis

Keywords

  • Angiogenesis
  • Aqueous humour cytokines
  • Fibroblast growth factor
  • Non-proliferative diabetic retinopathy
  • Optical coherence tomography
  • Retinal blood flow
  • Type 2 diabetes mellitus
  • Vasoregulatory

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Aqueous humour concentrations of TGF-β, PLGF and FGF-1 and total retinal blood flow in patients with early non-proliferative diabetic retinopathy. / Khuu, Lee Anne; Tayyari, Faryan; Sivak, Jeremy M.; Flanagan, John G.; Singer, Shaun; Brent, Michael H.; Huang, David; Tan, Ou; Hudson, Christopher.

In: Acta Ophthalmologica, 2016.

Research output: Contribution to journalArticle

Khuu, Lee Anne ; Tayyari, Faryan ; Sivak, Jeremy M. ; Flanagan, John G. ; Singer, Shaun ; Brent, Michael H. ; Huang, David ; Tan, Ou ; Hudson, Christopher. / Aqueous humour concentrations of TGF-β, PLGF and FGF-1 and total retinal blood flow in patients with early non-proliferative diabetic retinopathy. In: Acta Ophthalmologica. 2016.
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abstract = "Purpose: To correlate angiogenic cytokines in the aqueous humour with total retinal blood flow in subjects with type 2 diabetes with non-proliferative diabetic retinopathy (NPDR). Methods: A total of 17 controls and 16 NPDR patients were recruited into the study. Aqueous humour was collected at the start of cataract surgery to assess the concentration of 14 angiogenic cytokines. Aqueous humour was analysed using the suspension array method. Six images were acquired to assess total retinal blood flow (TRBF) using the prototype RTVue™ Doppler Fourier domain optical coherence tomography (Doppler FD-OCT) (Optovue, Inc., Fremont, CA) using a double circular scan protocol, 1 month postsurgery. At the same visit, forearm blood was collected to determine glycosylated haemoglobin (A1c). Results: Transforming growth factor beta (TGF-β1, TGF-β2) and PLGF were increased while FGF-1 was reduced in NPDR compared to controls (Bonferroni corrected, p < 0.003 for all). Total retinal blood flow (TRBF) was significantly reduced in the NPDR group compared to controls (33.1 ± 9.9 versus 43.3 ± 5.3 μl/min, p = 0.002). Aqueous FGF-1 significantly correlated with TRBF in the NPDR group (r = 0.71, p = 0.01; r2 = 0.51). In a multiple regression analysis, A1c was found to be a significant predictor of aqueous TGF-β1 and FGF-1 (p = 0.018 and p = 0.020, respectively). Conclusion: Aqueous angiogenic cytokines (TGF-β1, TGF-β2 and PLGF) were elevated in conjunction with a reduction in TRBF in patients with NPDR compared to controls. Non-invasive measurement of TRBF may be useful for predicting aqueous FGF-1 levels and severity of vasculopathy in DR.",
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T1 - Aqueous humour concentrations of TGF-β, PLGF and FGF-1 and total retinal blood flow in patients with early non-proliferative diabetic retinopathy

AU - Khuu, Lee Anne

AU - Tayyari, Faryan

AU - Sivak, Jeremy M.

AU - Flanagan, John G.

AU - Singer, Shaun

AU - Brent, Michael H.

AU - Huang, David

AU - Tan, Ou

AU - Hudson, Christopher

PY - 2016

Y1 - 2016

N2 - Purpose: To correlate angiogenic cytokines in the aqueous humour with total retinal blood flow in subjects with type 2 diabetes with non-proliferative diabetic retinopathy (NPDR). Methods: A total of 17 controls and 16 NPDR patients were recruited into the study. Aqueous humour was collected at the start of cataract surgery to assess the concentration of 14 angiogenic cytokines. Aqueous humour was analysed using the suspension array method. Six images were acquired to assess total retinal blood flow (TRBF) using the prototype RTVue™ Doppler Fourier domain optical coherence tomography (Doppler FD-OCT) (Optovue, Inc., Fremont, CA) using a double circular scan protocol, 1 month postsurgery. At the same visit, forearm blood was collected to determine glycosylated haemoglobin (A1c). Results: Transforming growth factor beta (TGF-β1, TGF-β2) and PLGF were increased while FGF-1 was reduced in NPDR compared to controls (Bonferroni corrected, p < 0.003 for all). Total retinal blood flow (TRBF) was significantly reduced in the NPDR group compared to controls (33.1 ± 9.9 versus 43.3 ± 5.3 μl/min, p = 0.002). Aqueous FGF-1 significantly correlated with TRBF in the NPDR group (r = 0.71, p = 0.01; r2 = 0.51). In a multiple regression analysis, A1c was found to be a significant predictor of aqueous TGF-β1 and FGF-1 (p = 0.018 and p = 0.020, respectively). Conclusion: Aqueous angiogenic cytokines (TGF-β1, TGF-β2 and PLGF) were elevated in conjunction with a reduction in TRBF in patients with NPDR compared to controls. Non-invasive measurement of TRBF may be useful for predicting aqueous FGF-1 levels and severity of vasculopathy in DR.

AB - Purpose: To correlate angiogenic cytokines in the aqueous humour with total retinal blood flow in subjects with type 2 diabetes with non-proliferative diabetic retinopathy (NPDR). Methods: A total of 17 controls and 16 NPDR patients were recruited into the study. Aqueous humour was collected at the start of cataract surgery to assess the concentration of 14 angiogenic cytokines. Aqueous humour was analysed using the suspension array method. Six images were acquired to assess total retinal blood flow (TRBF) using the prototype RTVue™ Doppler Fourier domain optical coherence tomography (Doppler FD-OCT) (Optovue, Inc., Fremont, CA) using a double circular scan protocol, 1 month postsurgery. At the same visit, forearm blood was collected to determine glycosylated haemoglobin (A1c). Results: Transforming growth factor beta (TGF-β1, TGF-β2) and PLGF were increased while FGF-1 was reduced in NPDR compared to controls (Bonferroni corrected, p < 0.003 for all). Total retinal blood flow (TRBF) was significantly reduced in the NPDR group compared to controls (33.1 ± 9.9 versus 43.3 ± 5.3 μl/min, p = 0.002). Aqueous FGF-1 significantly correlated with TRBF in the NPDR group (r = 0.71, p = 0.01; r2 = 0.51). In a multiple regression analysis, A1c was found to be a significant predictor of aqueous TGF-β1 and FGF-1 (p = 0.018 and p = 0.020, respectively). Conclusion: Aqueous angiogenic cytokines (TGF-β1, TGF-β2 and PLGF) were elevated in conjunction with a reduction in TRBF in patients with NPDR compared to controls. Non-invasive measurement of TRBF may be useful for predicting aqueous FGF-1 levels and severity of vasculopathy in DR.

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KW - Fibroblast growth factor

KW - Non-proliferative diabetic retinopathy

KW - Optical coherence tomography

KW - Retinal blood flow

KW - Type 2 diabetes mellitus

KW - Vasoregulatory

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