Application of novel vectors for CFP-tagging of proteins to study microtubule-associated proteins

Beat Ludin, Thierry Doll, Ruedi Meili, Stefanie Kaech, Andrew Matus

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

We describe the construction of pBact-NGFP and pBact-CGFP, two expression vectors that incorporate green fluorescent protein (GFP) as a fluorescent tag at the N- or C terminus of the produced protein. When transfected into recipient cells, GFP-tagged proteins can be visualised in the living cells using standard fluorescence microscopy techniques. Using these expression vectors, we have produced GFP-tagged versions of the neuronal microtubule-associated proteins (MAP), MAP2c and Tau34, in a number of different cell types. Both GFP-MAP2c and GFP-Tau34 were fluorescent and retained their ability to bind to microtubules. The pBact-NGFP and pBact-CGFP expression vectors represent a fast and convenient way to produce fluorescently tagged polypeptides of selected sequences encoding whole proteins or fragments for the analysis of function and dynamic events in living cells.

Original languageEnglish (US)
Pages (from-to)107-111
Number of pages5
JournalGene
Volume173
Issue number1
DOIs
StatePublished - Jan 1 1996

Keywords

  • Expression vector
  • Green fluorescent protein
  • MAP2
  • Microtubules
  • Tau protein

ASJC Scopus subject areas

  • Genetics

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