Abstract
We describe the construction of pBact-NGFP and pBact-CGFP, two expression vectors that incorporate green fluorescent protein (GFP) as a fluorescent tag at the N- or C terminus of the produced protein. When transfected into recipient cells, GFP-tagged proteins can be visualised in the living cells using standard fluorescence microscopy techniques. Using these expression vectors, we have produced GFP-tagged versions of the neuronal microtubule-associated proteins (MAP), MAP2c and Tau34, in a number of different cell types. Both GFP-MAP2c and GFP-Tau34 were fluorescent and retained their ability to bind to microtubules. The pBact-NGFP and pBact-CGFP expression vectors represent a fast and convenient way to produce fluorescently tagged polypeptides of selected sequences encoding whole proteins or fragments for the analysis of function and dynamic events in living cells.
Original language | English (US) |
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Pages (from-to) | 107-111 |
Number of pages | 5 |
Journal | Gene |
Volume | 173 |
Issue number | 1 |
DOIs | |
State | Published - 1996 |
Externally published | Yes |
Keywords
- Expression vector
- Green fluorescent protein
- MAP2
- Microtubules
- Tau protein
ASJC Scopus subject areas
- Genetics