Abstract
The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG-modified granulocyte-colony stimulating factor (PEG-G-CSF) prepared with PEG-aldehydes. The unmodified and PEG-modified G-CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size-based separation and quantitation of PEG-G-CSF. The Protein 80 Labchip was useful for PEG-5K-G-CSF, while the Protein 230 Labchip was more suitable for PEG-20K-G-CSF. The MCGE was also used to monitor a search for optimal PEG-modification (PEGylation) conditions to produce mono-PEG-G-CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G-CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.
Original language | English (US) |
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Pages (from-to) | 3771-3774 |
Number of pages | 4 |
Journal | ELECTROPHORESIS |
Volume | 31 |
Issue number | 22 |
DOIs | |
State | Published - Nov 2010 |
Externally published | Yes |
Keywords
- Granulocyte-colony stimulating factor
- Microchip CGE
- PEG-modification of protein
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry