Application of microchip CGE for the analysis of PEG-modified recombinant human granulocyte-colony stimulating factors

Eun Ji Park; Kyung Soo Lee; Kang Choon Lee; Dong Hee Na

doi:10.1002/elps.201000302

Application of microchip CGE for the analysis of PEG-modified recombinant human granulocyte-colony stimulating factors

Eun Ji Park, Kyung Soo Lee, Kang Choon Lee, Dong Hee Na

Pediatrics

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG-modified granulocyte-colony stimulating factor (PEG-G-CSF) prepared with PEG-aldehydes. The unmodified and PEG-modified G-CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size-based separation and quantitation of PEG-G-CSF. The Protein 80 Labchip was useful for PEG-5K-G-CSF, while the Protein 230 Labchip was more suitable for PEG-20K-G-CSF. The MCGE was also used to monitor a search for optimal PEG-modification (PEGylation) conditions to produce mono-PEG-G-CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G-CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.

Original language	English (US)
Pages (from-to)	3771-3774
Number of pages	4
Journal	ELECTROPHORESIS
Volume	31
Issue number	22
DOIs	https://doi.org/10.1002/elps.201000302
State	Published - Nov 1 2010

Keywords

Granulocyte-colony stimulating factor
Microchip CGE
PEG-modification of protein

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/elps.201000302

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title = "Application of microchip CGE for the analysis of PEG-modified recombinant human granulocyte-colony stimulating factors",

abstract = "The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG-modified granulocyte-colony stimulating factor (PEG-G-CSF) prepared with PEG-aldehydes. The unmodified and PEG-modified G-CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size-based separation and quantitation of PEG-G-CSF. The Protein 80 Labchip was useful for PEG-5K-G-CSF, while the Protein 230 Labchip was more suitable for PEG-20K-G-CSF. The MCGE was also used to monitor a search for optimal PEG-modification (PEGylation) conditions to produce mono-PEG-G-CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G-CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.",

keywords = "Granulocyte-colony stimulating factor, Microchip CGE, PEG-modification of protein",

author = "Park, {Eun Ji} and Lee, {Kyung Soo} and Lee, {Kang Choon} and Na, {Dong Hee}",

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doi = "10.1002/elps.201000302",

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AU - Park, Eun Ji

AU - Lee, Kyung Soo

AU - Lee, Kang Choon

AU - Na, Dong Hee

PY - 2010/11/1

Y1 - 2010/11/1

N2 - The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG-modified granulocyte-colony stimulating factor (PEG-G-CSF) prepared with PEG-aldehydes. The unmodified and PEG-modified G-CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size-based separation and quantitation of PEG-G-CSF. The Protein 80 Labchip was useful for PEG-5K-G-CSF, while the Protein 230 Labchip was more suitable for PEG-20K-G-CSF. The MCGE was also used to monitor a search for optimal PEG-modification (PEGylation) conditions to produce mono-PEG-G-CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G-CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.

AB - The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG-modified granulocyte-colony stimulating factor (PEG-G-CSF) prepared with PEG-aldehydes. The unmodified and PEG-modified G-CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size-based separation and quantitation of PEG-G-CSF. The Protein 80 Labchip was useful for PEG-5K-G-CSF, while the Protein 230 Labchip was more suitable for PEG-20K-G-CSF. The MCGE was also used to monitor a search for optimal PEG-modification (PEGylation) conditions to produce mono-PEG-G-CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G-CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.

KW - Granulocyte-colony stimulating factor

KW - Microchip CGE

KW - PEG-modification of protein

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Application of microchip CGE for the analysis of PEG-modified recombinant human granulocyte-colony stimulating factors

Abstract

Keywords

ASJC Scopus subject areas

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