Application of microchip CGE for the analysis of PEG-modified recombinant human granulocyte-colony stimulating factors

Eun Ji Park, Soo-Kyung Lee, Kang Choon Lee, Dong Hee Na

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG-modified granulocyte-colony stimulating factor (PEG-G-CSF) prepared with PEG-aldehydes. The unmodified and PEG-modified G-CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size-based separation and quantitation of PEG-G-CSF. The Protein 80 Labchip was useful for PEG-5K-G-CSF, while the Protein 230 Labchip was more suitable for PEG-20K-G-CSF. The MCGE was also used to monitor a search for optimal PEG-modification (PEGylation) conditions to produce mono-PEG-G-CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G-CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.

Original languageEnglish (US)
Pages (from-to)3771-3774
Number of pages4
JournalElectrophoresis
Volume31
Issue number22
DOIs
StatePublished - Nov 2010
Externally publishedYes

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Microchip Analytical Procedures
Granulocyte Colony-Stimulating Factor
Polyethylene glycols
Proteins
Aldehydes

Keywords

  • Granulocyte-colony stimulating factor
  • Microchip CGE
  • PEG-modification of protein

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Application of microchip CGE for the analysis of PEG-modified recombinant human granulocyte-colony stimulating factors. / Park, Eun Ji; Lee, Soo-Kyung; Lee, Kang Choon; Na, Dong Hee.

In: Electrophoresis, Vol. 31, No. 22, 11.2010, p. 3771-3774.

Research output: Contribution to journalArticle

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