Application of microchip CGE for the analysis of PEG-modified recombinant human granulocyte-colony stimulating factors

Eun Ji Park, Kyung Soo Lee, Kang Choon Lee, Dong Hee Na

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG-modified granulocyte-colony stimulating factor (PEG-G-CSF) prepared with PEG-aldehydes. The unmodified and PEG-modified G-CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size-based separation and quantitation of PEG-G-CSF. The Protein 80 Labchip was useful for PEG-5K-G-CSF, while the Protein 230 Labchip was more suitable for PEG-20K-G-CSF. The MCGE was also used to monitor a search for optimal PEG-modification (PEGylation) conditions to produce mono-PEG-G-CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G-CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.

Original languageEnglish (US)
Pages (from-to)3771-3774
Number of pages4
JournalELECTROPHORESIS
Volume31
Issue number22
DOIs
StatePublished - Nov 1 2010

Keywords

  • Granulocyte-colony stimulating factor
  • Microchip CGE
  • PEG-modification of protein

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Application of microchip CGE for the analysis of PEG-modified recombinant human granulocyte-colony stimulating factors'. Together they form a unique fingerprint.

  • Cite this