Abstract
The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG-modified granulocyte-colony stimulating factor (PEG-G-CSF) prepared with PEG-aldehydes. The unmodified and PEG-modified G-CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size-based separation and quantitation of PEG-G-CSF. The Protein 80 Labchip was useful for PEG-5K-G-CSF, while the Protein 230 Labchip was more suitable for PEG-20K-G-CSF. The MCGE was also used to monitor a search for optimal PEG-modification (PEGylation) conditions to produce mono-PEG-G-CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G-CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3771-3774 |
| Number of pages | 4 |
| Journal | Electrophoresis |
| Volume | 31 |
| Issue number | 22 |
| DOIs | |
| State | Published - Nov 2010 |
| Externally published | Yes |
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Keywords
- Granulocyte-colony stimulating factor
- Microchip CGE
- PEG-modification of protein
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
Cite this
Application of microchip CGE for the analysis of PEG-modified recombinant human granulocyte-colony stimulating factors. / Park, Eun Ji; Lee, Soo-Kyung; Lee, Kang Choon; Na, Dong Hee.
In: Electrophoresis, Vol. 31, No. 22, 11.2010, p. 3771-3774.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Application of microchip CGE for the analysis of PEG-modified recombinant human granulocyte-colony stimulating factors
AU - Park, Eun Ji
AU - Lee, Soo-Kyung
AU - Lee, Kang Choon
AU - Na, Dong Hee
PY - 2010/11
Y1 - 2010/11
N2 - The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG-modified granulocyte-colony stimulating factor (PEG-G-CSF) prepared with PEG-aldehydes. The unmodified and PEG-modified G-CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size-based separation and quantitation of PEG-G-CSF. The Protein 80 Labchip was useful for PEG-5K-G-CSF, while the Protein 230 Labchip was more suitable for PEG-20K-G-CSF. The MCGE was also used to monitor a search for optimal PEG-modification (PEGylation) conditions to produce mono-PEG-G-CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G-CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.
AB - The purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG-modified granulocyte-colony stimulating factor (PEG-G-CSF) prepared with PEG-aldehydes. The unmodified and PEG-modified G-CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size-based separation and quantitation of PEG-G-CSF. The Protein 80 Labchip was useful for PEG-5K-G-CSF, while the Protein 230 Labchip was more suitable for PEG-20K-G-CSF. The MCGE was also used to monitor a search for optimal PEG-modification (PEGylation) conditions to produce mono-PEG-G-CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G-CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.
KW - Granulocyte-colony stimulating factor
KW - Microchip CGE
KW - PEG-modification of protein
UR - http://www.scopus.com/inward/record.url?scp=78449250965&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78449250965&partnerID=8YFLogxK
U2 - 10.1002/elps.201000302
DO - 10.1002/elps.201000302
M3 - Article
C2 - 20945411
AN - SCOPUS:78449250965
VL - 31
SP - 3771
EP - 3774
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 22
ER -