Application of matrix solid-phase dispersion in the determination of dibenzo[a,l]pyrene content of experimental animal diets used in a large-scale tumor study

P. M. Loveland, A. P. Reddy, C. B. Pereira, J. A. Field, G. S. Bailey

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A method utilizing matrix solid-phase dispersion (MSPD) was developed for isolation and determination of dibenzo[a,l]pyrene (DBP) in experimental rainbow-trout diets used in a large-scale carcinogenesis study. A 0.5 g sample of moist ration containing 0-225 ppm DBP (dry basis) was mixed with 2 g C18 sorbent and benzo[a]pyrene internal standard was added to the mixture. Extraction and clean-up were accomplished in a single step by extracting the sample mixture with hexane-benzene 4:1 from a cartridge containing 2 g Florisil®. DBP was quantified by HPLC on a C8 bonded phase column with fluorescence detection. Mean analytical recovery of DBP from control diet spiked at three concentration levels was 101 to 107% with relative standard deviations of 1 to 7%. The limit of detection of DBP was equivalent to 0.014 ppm in the ration. Application of the method to verification of DBP levels in trout rations from the carcinogenesis study is described. Control ration (0 ppm DBP) was screened for possible DBP contamination and none was found. This is the first report on analysis of DBP in experimental animal diets.

Original languageEnglish (US)
Pages (from-to)33-41
Number of pages9
JournalJournal of Chromatography A
Volume932
Issue number1-2
DOIs
StatePublished - Oct 12 2001
Externally publishedYes

Keywords

  • Dibenzo[a,l]pyrene
  • Matrix solid-phase dispersion

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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