Application of adenosine triphosphate-driven bioluminescence for quantification of plaque bacteria and assessment of oral hygiene in children

Shahram Fazilat, Rebecca Sauerwein, Jennifer McLeod, Tyler Finlayson, Emilia Adam, John Engle, Prashant Gagneja, Tom Maier, Curtis Machida

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Purpose: Dentistry has undergone a shift in caries management toward prevention and improved oral hygiene and diagnosis. Caries prevention now represents one of the most important aspects of modern dental practice. The purpose of this cross-sectional study was to demonstrate the use of adenosine triphosphate- (ATP-) driven bioluminescence as an innovative tool for the rapid chairside enumeration of oral bacteria (including plaque streptococci) and assessment of oral hygiene and caries risk. Methods: Thirty-three pediatric patients (7- to 12-year-old males and females) were examined, and plaque specimens, in addition to stimulated saliva, were collected from representative teeth within each quadrant. Oral specimens (n=150 specimens) were assessed by plating on enriched and selective agars, to enumerate total bacteria and streptococci, and subjected to adenosine triphosphate- (ATP-) driven bioluminescence determinations using a luciferase-based assay system. Results: Statistical correlations, linking ATP values to numbers of total bacteria, oral streptococci and mutans streptococci, yielded highly significant r values of 0.854, 0.840, and 0.796, respectively. Conclusions: Our clinical data is consistent with the hypothesis that ATP measurements have a strong statistical association with bacterial number in plaque and saliva specimens, including numbers for oral streptococci, and may be used as a potential assessment tool for oral hygiene ana caries risk in children.

Original languageEnglish (US)
Pages (from-to)195-204
Number of pages10
JournalPediatric Dentistry
Volume32
Issue number3
StatePublished - May 2010

Fingerprint

Oral Hygiene
Streptococcus
Adenosine Triphosphate
Bacteria
Saliva
Tooth
Oral Diagnosis
Streptococcus mutans
Dentistry
Luciferases
Agar
Cross-Sectional Studies
Pediatrics

Keywords

  • ATP-driven bioluminescence
  • Caries risk assessment
  • Oral hygiene
  • Pediatric patients
  • Plaque bacteria

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Application of adenosine triphosphate-driven bioluminescence for quantification of plaque bacteria and assessment of oral hygiene in children. / Fazilat, Shahram; Sauerwein, Rebecca; McLeod, Jennifer; Finlayson, Tyler; Adam, Emilia; Engle, John; Gagneja, Prashant; Maier, Tom; Machida, Curtis.

In: Pediatric Dentistry, Vol. 32, No. 3, 05.2010, p. 195-204.

Research output: Contribution to journalArticle

Fazilat, Shahram ; Sauerwein, Rebecca ; McLeod, Jennifer ; Finlayson, Tyler ; Adam, Emilia ; Engle, John ; Gagneja, Prashant ; Maier, Tom ; Machida, Curtis. / Application of adenosine triphosphate-driven bioluminescence for quantification of plaque bacteria and assessment of oral hygiene in children. In: Pediatric Dentistry. 2010 ; Vol. 32, No. 3. pp. 195-204.
@article{8aed95ca449c43589eb69f90ac936bfd,
title = "Application of adenosine triphosphate-driven bioluminescence for quantification of plaque bacteria and assessment of oral hygiene in children",
abstract = "Purpose: Dentistry has undergone a shift in caries management toward prevention and improved oral hygiene and diagnosis. Caries prevention now represents one of the most important aspects of modern dental practice. The purpose of this cross-sectional study was to demonstrate the use of adenosine triphosphate- (ATP-) driven bioluminescence as an innovative tool for the rapid chairside enumeration of oral bacteria (including plaque streptococci) and assessment of oral hygiene and caries risk. Methods: Thirty-three pediatric patients (7- to 12-year-old males and females) were examined, and plaque specimens, in addition to stimulated saliva, were collected from representative teeth within each quadrant. Oral specimens (n=150 specimens) were assessed by plating on enriched and selective agars, to enumerate total bacteria and streptococci, and subjected to adenosine triphosphate- (ATP-) driven bioluminescence determinations using a luciferase-based assay system. Results: Statistical correlations, linking ATP values to numbers of total bacteria, oral streptococci and mutans streptococci, yielded highly significant r values of 0.854, 0.840, and 0.796, respectively. Conclusions: Our clinical data is consistent with the hypothesis that ATP measurements have a strong statistical association with bacterial number in plaque and saliva specimens, including numbers for oral streptococci, and may be used as a potential assessment tool for oral hygiene ana caries risk in children.",
keywords = "ATP-driven bioluminescence, Caries risk assessment, Oral hygiene, Pediatric patients, Plaque bacteria",
author = "Shahram Fazilat and Rebecca Sauerwein and Jennifer McLeod and Tyler Finlayson and Emilia Adam and John Engle and Prashant Gagneja and Tom Maier and Curtis Machida",
year = "2010",
month = "5",
language = "English (US)",
volume = "32",
pages = "195--204",
journal = "Pediatric Dentistry",
issn = "0164-1263",
publisher = "American Academy of Pediatric Dentistry",
number = "3",

}

TY - JOUR

T1 - Application of adenosine triphosphate-driven bioluminescence for quantification of plaque bacteria and assessment of oral hygiene in children

AU - Fazilat, Shahram

AU - Sauerwein, Rebecca

AU - McLeod, Jennifer

AU - Finlayson, Tyler

AU - Adam, Emilia

AU - Engle, John

AU - Gagneja, Prashant

AU - Maier, Tom

AU - Machida, Curtis

PY - 2010/5

Y1 - 2010/5

N2 - Purpose: Dentistry has undergone a shift in caries management toward prevention and improved oral hygiene and diagnosis. Caries prevention now represents one of the most important aspects of modern dental practice. The purpose of this cross-sectional study was to demonstrate the use of adenosine triphosphate- (ATP-) driven bioluminescence as an innovative tool for the rapid chairside enumeration of oral bacteria (including plaque streptococci) and assessment of oral hygiene and caries risk. Methods: Thirty-three pediatric patients (7- to 12-year-old males and females) were examined, and plaque specimens, in addition to stimulated saliva, were collected from representative teeth within each quadrant. Oral specimens (n=150 specimens) were assessed by plating on enriched and selective agars, to enumerate total bacteria and streptococci, and subjected to adenosine triphosphate- (ATP-) driven bioluminescence determinations using a luciferase-based assay system. Results: Statistical correlations, linking ATP values to numbers of total bacteria, oral streptococci and mutans streptococci, yielded highly significant r values of 0.854, 0.840, and 0.796, respectively. Conclusions: Our clinical data is consistent with the hypothesis that ATP measurements have a strong statistical association with bacterial number in plaque and saliva specimens, including numbers for oral streptococci, and may be used as a potential assessment tool for oral hygiene ana caries risk in children.

AB - Purpose: Dentistry has undergone a shift in caries management toward prevention and improved oral hygiene and diagnosis. Caries prevention now represents one of the most important aspects of modern dental practice. The purpose of this cross-sectional study was to demonstrate the use of adenosine triphosphate- (ATP-) driven bioluminescence as an innovative tool for the rapid chairside enumeration of oral bacteria (including plaque streptococci) and assessment of oral hygiene and caries risk. Methods: Thirty-three pediatric patients (7- to 12-year-old males and females) were examined, and plaque specimens, in addition to stimulated saliva, were collected from representative teeth within each quadrant. Oral specimens (n=150 specimens) were assessed by plating on enriched and selective agars, to enumerate total bacteria and streptococci, and subjected to adenosine triphosphate- (ATP-) driven bioluminescence determinations using a luciferase-based assay system. Results: Statistical correlations, linking ATP values to numbers of total bacteria, oral streptococci and mutans streptococci, yielded highly significant r values of 0.854, 0.840, and 0.796, respectively. Conclusions: Our clinical data is consistent with the hypothesis that ATP measurements have a strong statistical association with bacterial number in plaque and saliva specimens, including numbers for oral streptococci, and may be used as a potential assessment tool for oral hygiene ana caries risk in children.

KW - ATP-driven bioluminescence

KW - Caries risk assessment

KW - Oral hygiene

KW - Pediatric patients

KW - Plaque bacteria

UR - http://www.scopus.com/inward/record.url?scp=77955080272&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955080272&partnerID=8YFLogxK

M3 - Article

C2 - 20557702

AN - SCOPUS:77955080272

VL - 32

SP - 195

EP - 204

JO - Pediatric Dentistry

JF - Pediatric Dentistry

SN - 0164-1263

IS - 3

ER -