Application of a patient derived xenograft model for predicative study of uterine fibroid disease

Martin Fritsch, Nicole Schmidt, Ina Gröticke, Anna Lena Frisk, Christopher S. Keator, Markus Koch, Ov Slayden

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Human uterine fibroids, benign tumors derived from the smooth muscle layers of the uterus, impose a major health burden to up to 50% of premenopausal women in their daily life. To improve our understanding of this disease, we developed and characterized a patientderived xenograft model by subcutaneous transplantation of pieces of human uterine fibroid tissue into three different strains of severe combined immunodeficient mice. Engrafted uterine fibroid tissue preserved the classical morphology with interwoven bundles of smooth muscle cells and an abundant deposition of collagenous matrix, similar to uterine fibroids in situ. The grafts expressed both estrogen receptor 1 and progesterone receptor. Additionally, both receptors were up-regulated by estrogen treatment. Growth of the fibroid grafts was dependent on 17β-estradiol and progesterone supplementation at levels similar to women with the disease and was studied for up to 60 days at maximum. Co-treatment with the antiprogestin mifepristone reduced graft growth (four independent donors, p

    Original languageEnglish (US)
    Article numbere0142429
    JournalPLoS One
    Volume10
    Issue number11
    DOIs
    StatePublished - Nov 1 2015

    Fingerprint

    uterine diseases
    Uterine Diseases
    uterine tissue
    Leiomyoma
    Heterografts
    Grafts
    smooth muscle
    Muscle
    myocytes
    uterus
    Tissue
    estrogens
    estradiol
    Mifepristone
    progesterone
    Estrogen Receptor alpha
    Progesterone Receptors
    Transplants
    receptors
    neoplasms

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Fritsch, M., Schmidt, N., Gröticke, I., Frisk, A. L., Keator, C. S., Koch, M., & Slayden, O. (2015). Application of a patient derived xenograft model for predicative study of uterine fibroid disease. PLoS One, 10(11), [e0142429]. https://doi.org/10.1371/journal.pone.0142429

    Application of a patient derived xenograft model for predicative study of uterine fibroid disease. / Fritsch, Martin; Schmidt, Nicole; Gröticke, Ina; Frisk, Anna Lena; Keator, Christopher S.; Koch, Markus; Slayden, Ov.

    In: PLoS One, Vol. 10, No. 11, e0142429, 01.11.2015.

    Research output: Contribution to journalArticle

    Fritsch, M, Schmidt, N, Gröticke, I, Frisk, AL, Keator, CS, Koch, M & Slayden, O 2015, 'Application of a patient derived xenograft model for predicative study of uterine fibroid disease', PLoS One, vol. 10, no. 11, e0142429. https://doi.org/10.1371/journal.pone.0142429
    Fritsch M, Schmidt N, Gröticke I, Frisk AL, Keator CS, Koch M et al. Application of a patient derived xenograft model for predicative study of uterine fibroid disease. PLoS One. 2015 Nov 1;10(11). e0142429. https://doi.org/10.1371/journal.pone.0142429
    Fritsch, Martin ; Schmidt, Nicole ; Gröticke, Ina ; Frisk, Anna Lena ; Keator, Christopher S. ; Koch, Markus ; Slayden, Ov. / Application of a patient derived xenograft model for predicative study of uterine fibroid disease. In: PLoS One. 2015 ; Vol. 10, No. 11.
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