Apoptotic signaling proteins: Possible participation in the regulation of vasopressin and catecholamines biosynthesis in the hypothalamus

Elena V. Chernigovskaya, Andrey G. Taranukhin, Margarita V. Glazova, Liubov A. Yamova, Lev M. Fedorov

    Research output: Contribution to journalArticle

    21 Scopus citations

    Abstract

    The role of apoptotic signaling proteins for long-lived neurons in the mature brain is poorly understood. Recently, we have shown that water deprivation leads to the activation of vasopressin (VP) secretion and expression of Bcl-2 and caspase-9 apototic proteins in the hypothalamus of the rat brain. In the present work, we continued to study a possible relationship between the functional activity of neurosecretory cells of the hypothalamus and apoptosis related proteins. We found that water deprivation leads to simultaneous activation of synthesis of VP and p53 and Bcl-2 apoptotic proteins in the mouse brain. To study a possible effect of apoptotic proteins on the functional state of hypothalamic neurons, the VP and tyrosine hydroxylase (TH) synthesis were analyzed in p53, p21Waf1/Cip1 and Bcl-2 deficient mice. Loss of p53 and Bcl-2 significantly reduced VP synthesis in paraventricular and supraoptic nuclei and TH expression in arcuat, periventricular and zona incerta nuclei of the hypothalamus. Surprisingly, in contrast with the loss of p53, the inactivation of p21Waf1/Cip1 up-regulates the expression of VP and TH. These data indicate that p53, p21Waf1/Cip1 and Bcl-2 proteins, besides affecting cell cycle, tumor suppression and apoptosis, may act as modulators of neurosecretory activity of hypothalamic neurons; however, this problem remains to be determined more detailed.

    Original languageEnglish (US)
    Pages (from-to)523-533
    Number of pages11
    JournalHistochemistry and Cell Biology
    Volume124
    Issue number6
    DOIs
    StatePublished - Dec 2005

    Keywords

    • Apoptosis
    • Bcl-2
    • Hypothalamus
    • Vasopressin
    • p53

    ASJC Scopus subject areas

    • Histology
    • Molecular Biology
    • Medical Laboratory Technology
    • Cell Biology

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