Apoptosis induced in L1210 leukaemia cells by an inhibitor of the chymotrypsin-like activity of the proteasome

Cezary Wojcik, T. Stoklosa, A. Giermasz, J. Golab, R. Zagozdzon, J. Kawiak, S. Wilk, A. Komar, A. Kaca, J. Malejczyk, M. Jakóbisiak

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Of a number of factors involved in apoptosis, protease activity may play a crucial role. We show that N-benzyloxycarbonyl-IIe-Glu(O-t-butyl)-Ala-leucinal (PSI), a selective inhibitor of the chymotrypsin-like activity of the proteasome, induces massive apoptosis in murine leukaemia L1210 cells. At 50 nM concentration, PSI induces a block of cytokinesis, while higher concentrations (500 nM) cause S phase block and massive apoptosis. Z-Leu-leucinal, a specific calpain inhibitor, did not induce apoptosis. In contrast to previous reports, TNF-α did not enhance apoptosis when combined with PSI. Our results suggest that proteasome inhibitors may be considered as potential anti-neoplastic agents.

Original languageEnglish (US)
Pages (from-to)455-462
Number of pages8
JournalApoptosis
Volume2
Issue number5
StatePublished - 1997
Externally publishedYes

Fingerprint

Leukemia L1210
Chymotrypsin
Proteasome Endopeptidase Complex
Apoptosis
Proteasome Inhibitors
Cytokinesis
S Phase
Peptide Hydrolases

Keywords

  • Apoptosis
  • Experimental cancer therapy
  • L1210 leukaemia
  • Proteasome, proteasome inhibitor, TNF

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Wojcik, C., Stoklosa, T., Giermasz, A., Golab, J., Zagozdzon, R., Kawiak, J., ... Jakóbisiak, M. (1997). Apoptosis induced in L1210 leukaemia cells by an inhibitor of the chymotrypsin-like activity of the proteasome. Apoptosis, 2(5), 455-462.

Apoptosis induced in L1210 leukaemia cells by an inhibitor of the chymotrypsin-like activity of the proteasome. / Wojcik, Cezary; Stoklosa, T.; Giermasz, A.; Golab, J.; Zagozdzon, R.; Kawiak, J.; Wilk, S.; Komar, A.; Kaca, A.; Malejczyk, J.; Jakóbisiak, M.

In: Apoptosis, Vol. 2, No. 5, 1997, p. 455-462.

Research output: Contribution to journalArticle

Wojcik, C, Stoklosa, T, Giermasz, A, Golab, J, Zagozdzon, R, Kawiak, J, Wilk, S, Komar, A, Kaca, A, Malejczyk, J & Jakóbisiak, M 1997, 'Apoptosis induced in L1210 leukaemia cells by an inhibitor of the chymotrypsin-like activity of the proteasome', Apoptosis, vol. 2, no. 5, pp. 455-462.
Wojcik C, Stoklosa T, Giermasz A, Golab J, Zagozdzon R, Kawiak J et al. Apoptosis induced in L1210 leukaemia cells by an inhibitor of the chymotrypsin-like activity of the proteasome. Apoptosis. 1997;2(5):455-462.
Wojcik, Cezary ; Stoklosa, T. ; Giermasz, A. ; Golab, J. ; Zagozdzon, R. ; Kawiak, J. ; Wilk, S. ; Komar, A. ; Kaca, A. ; Malejczyk, J. ; Jakóbisiak, M. / Apoptosis induced in L1210 leukaemia cells by an inhibitor of the chymotrypsin-like activity of the proteasome. In: Apoptosis. 1997 ; Vol. 2, No. 5. pp. 455-462.
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