Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization

David Seifer, A. C. Cardiner, K. A. Ferreira, J. J. Peluso

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Objective: To test the hypothesis that preovulatory follicles from women with diminished ovarian reserve contain fewer numbers of luteinized granulosa cells and have a greater percentage of cells undergoing apoptosis as compared with women with uncompromised ovarian reserve undergoing superovulation for IVF-ET. Design: Comparative study. Setting: Academic clinical practice. Patients: Luteinized granulosa cell counts were determined from follicular aspirates harvested from 19 women with day 3 serum FSH ≤ 6 mIU/mL (conversion factor to SI unit, 1.00) and from 15 women with FSH levels ≤ 10 mIU/mL. The second part examined the percent of proliferating and apoptotic cells harvested from nine women with day 3 serum FSH ≤ 6 mIU/mL and from eight women with FSH levels ≤ 10 mIU/mL. Main Outcome Measures: Cell counts by hemacytometer and percent of proliferating and of apoptotic granulosa cells by flow cytometry. Results: The mean number of viable cells isolated per follicle were 2.7 x 105 (95% confidence interval [CI] 2.3 to 3.1 x 105) from the low FSH group and 1.8 ~ 105 (95% CI 1.4 to 2.3 x 105) from the high FSH group. Although the percentage of proliferating cells was the same, the mean percentage of apoptotic cells was 0.5% (95% CI 0.2% to 1.4%) from the low group and 2.0% (95% CI 1.1% to 3.7%) from the high group. Conclusions: Women undergoing IVF with day 3 serum FSkI ≤ 10 mIU/mL have preovulatory follicles with fewer luteinized granulosa cells and an increase in the percentage of cells undergoing apoptosis as compared with women with day 3 serum FSH ≤ 6 mIU/mL.

Original languageEnglish (US)
Pages (from-to)593-598
Number of pages6
JournalFertility and Sterility
Volume66
Issue number4
StatePublished - 1996
Externally publishedYes

Fingerprint

Fertilization in Vitro
Apoptosis
Granulosa Cells
Confidence Intervals
Cell Count
Serum
International System of Units
Superovulation
Ovarian Reserve
Flow Cytometry
Outcome Assessment (Health Care)

Keywords

  • apoptosis
  • flow cytometry
  • Granulosa cells
  • ovarian reserve

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Seifer, D., Cardiner, A. C., Ferreira, K. A., & Peluso, J. J. (1996). Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization. Fertility and Sterility, 66(4), 593-598.

Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization. / Seifer, David; Cardiner, A. C.; Ferreira, K. A.; Peluso, J. J.

In: Fertility and Sterility, Vol. 66, No. 4, 1996, p. 593-598.

Research output: Contribution to journalArticle

Seifer, D, Cardiner, AC, Ferreira, KA & Peluso, JJ 1996, 'Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization', Fertility and Sterility, vol. 66, no. 4, pp. 593-598.
Seifer, David ; Cardiner, A. C. ; Ferreira, K. A. ; Peluso, J. J. / Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization. In: Fertility and Sterility. 1996 ; Vol. 66, No. 4. pp. 593-598.
@article{e46e080470fb4984b3808fce72e8e769,
title = "Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization",
abstract = "Objective: To test the hypothesis that preovulatory follicles from women with diminished ovarian reserve contain fewer numbers of luteinized granulosa cells and have a greater percentage of cells undergoing apoptosis as compared with women with uncompromised ovarian reserve undergoing superovulation for IVF-ET. Design: Comparative study. Setting: Academic clinical practice. Patients: Luteinized granulosa cell counts were determined from follicular aspirates harvested from 19 women with day 3 serum FSH ≤ 6 mIU/mL (conversion factor to SI unit, 1.00) and from 15 women with FSH levels ≤ 10 mIU/mL. The second part examined the percent of proliferating and apoptotic cells harvested from nine women with day 3 serum FSH ≤ 6 mIU/mL and from eight women with FSH levels ≤ 10 mIU/mL. Main Outcome Measures: Cell counts by hemacytometer and percent of proliferating and of apoptotic granulosa cells by flow cytometry. Results: The mean number of viable cells isolated per follicle were 2.7 x 105 (95{\%} confidence interval [CI] 2.3 to 3.1 x 105) from the low FSH group and 1.8 ~ 105 (95{\%} CI 1.4 to 2.3 x 105) from the high FSH group. Although the percentage of proliferating cells was the same, the mean percentage of apoptotic cells was 0.5{\%} (95{\%} CI 0.2{\%} to 1.4{\%}) from the low group and 2.0{\%} (95{\%} CI 1.1{\%} to 3.7{\%}) from the high group. Conclusions: Women undergoing IVF with day 3 serum FSkI ≤ 10 mIU/mL have preovulatory follicles with fewer luteinized granulosa cells and an increase in the percentage of cells undergoing apoptosis as compared with women with day 3 serum FSH ≤ 6 mIU/mL.",
keywords = "apoptosis, flow cytometry, Granulosa cells, ovarian reserve",
author = "David Seifer and Cardiner, {A. C.} and Ferreira, {K. A.} and Peluso, {J. J.}",
year = "1996",
language = "English (US)",
volume = "66",
pages = "593--598",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization

AU - Seifer, David

AU - Cardiner, A. C.

AU - Ferreira, K. A.

AU - Peluso, J. J.

PY - 1996

Y1 - 1996

N2 - Objective: To test the hypothesis that preovulatory follicles from women with diminished ovarian reserve contain fewer numbers of luteinized granulosa cells and have a greater percentage of cells undergoing apoptosis as compared with women with uncompromised ovarian reserve undergoing superovulation for IVF-ET. Design: Comparative study. Setting: Academic clinical practice. Patients: Luteinized granulosa cell counts were determined from follicular aspirates harvested from 19 women with day 3 serum FSH ≤ 6 mIU/mL (conversion factor to SI unit, 1.00) and from 15 women with FSH levels ≤ 10 mIU/mL. The second part examined the percent of proliferating and apoptotic cells harvested from nine women with day 3 serum FSH ≤ 6 mIU/mL and from eight women with FSH levels ≤ 10 mIU/mL. Main Outcome Measures: Cell counts by hemacytometer and percent of proliferating and of apoptotic granulosa cells by flow cytometry. Results: The mean number of viable cells isolated per follicle were 2.7 x 105 (95% confidence interval [CI] 2.3 to 3.1 x 105) from the low FSH group and 1.8 ~ 105 (95% CI 1.4 to 2.3 x 105) from the high FSH group. Although the percentage of proliferating cells was the same, the mean percentage of apoptotic cells was 0.5% (95% CI 0.2% to 1.4%) from the low group and 2.0% (95% CI 1.1% to 3.7%) from the high group. Conclusions: Women undergoing IVF with day 3 serum FSkI ≤ 10 mIU/mL have preovulatory follicles with fewer luteinized granulosa cells and an increase in the percentage of cells undergoing apoptosis as compared with women with day 3 serum FSH ≤ 6 mIU/mL.

AB - Objective: To test the hypothesis that preovulatory follicles from women with diminished ovarian reserve contain fewer numbers of luteinized granulosa cells and have a greater percentage of cells undergoing apoptosis as compared with women with uncompromised ovarian reserve undergoing superovulation for IVF-ET. Design: Comparative study. Setting: Academic clinical practice. Patients: Luteinized granulosa cell counts were determined from follicular aspirates harvested from 19 women with day 3 serum FSH ≤ 6 mIU/mL (conversion factor to SI unit, 1.00) and from 15 women with FSH levels ≤ 10 mIU/mL. The second part examined the percent of proliferating and apoptotic cells harvested from nine women with day 3 serum FSH ≤ 6 mIU/mL and from eight women with FSH levels ≤ 10 mIU/mL. Main Outcome Measures: Cell counts by hemacytometer and percent of proliferating and of apoptotic granulosa cells by flow cytometry. Results: The mean number of viable cells isolated per follicle were 2.7 x 105 (95% confidence interval [CI] 2.3 to 3.1 x 105) from the low FSH group and 1.8 ~ 105 (95% CI 1.4 to 2.3 x 105) from the high FSH group. Although the percentage of proliferating cells was the same, the mean percentage of apoptotic cells was 0.5% (95% CI 0.2% to 1.4%) from the low group and 2.0% (95% CI 1.1% to 3.7%) from the high group. Conclusions: Women undergoing IVF with day 3 serum FSkI ≤ 10 mIU/mL have preovulatory follicles with fewer luteinized granulosa cells and an increase in the percentage of cells undergoing apoptosis as compared with women with day 3 serum FSH ≤ 6 mIU/mL.

KW - apoptosis

KW - flow cytometry

KW - Granulosa cells

KW - ovarian reserve

UR - http://www.scopus.com/inward/record.url?scp=0029810496&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029810496&partnerID=8YFLogxK

M3 - Article

VL - 66

SP - 593

EP - 598

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 4

ER -