Apo-AII is an elevated biomaker of chronic non-human primate ethanol self-administration

Willard M. Freeman, Randy S. Gooch, Melinda E. Lull, Travis J. Worst, Stephen J. Walker, Arron S L Xu, Heather Green, Peter J. Pierre, Kathleen (Kathy) Grant, Kent E. Vrana

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aims: Serum protein profiles were examined in näve, ethanol self-administering and ethanol abstinent cynomolgus monkeys (Macaca fasicularis) to search for differences in protein expression which could possibly serve as biomarkers of heavy ethanol consumption. Methods: Surface-enhanced laser desorption ionization time-of-flight (SELDI-ToF) mass spectrometry was used for proteomic profiling of serum. Results: Two proteins were identified by SELDI-ToF to be increased in ethanol self-administering compared with abstinent animals. These proteins were identified to be apolipoprotein AI (Apo-AI) and apolipoprotein AII (Apo-AII) by peptide mass fingerprinting and comparison with spectra of purified human Apo-AI and AII proteins. Immunoblot analysis of Apo-AI and Apo-AII was performed on a separate group of animals (within-animal ethanol-näve and self-administering) and confirmed a statistically significant increase in Apo-AII, while Apo-AI was unchanged. Conclusions: An open proteomic screen of serum and confirmation in a separate set of animals found Apo-AII to be increased in the serum of ethanol self-administering monkeys. These results are consistent with previous clinical studies of human ethanol consumption and serum apolipoprotein expression. Moreover, these results validate the use of non-human primates as a model organism for proteomic analysis of ethanol self-administration biomarkers.

Original languageEnglish (US)
Pages (from-to)300-305
Number of pages6
JournalAlcohol and Alcoholism
Volume41
Issue number3
DOIs
StatePublished - May 2006
Externally publishedYes

Fingerprint

Apolipoprotein A-II
Self Administration
Primates
Ethanol
Apolipoprotein A-I
Animals
Proteomics
Biomarkers
Serum
Ionization
Desorption
Proteins
Lasers
Peptide Mapping
Macaca fascicularis
Apolipoproteins
Macaca
Haplorhini
Mass spectrometry
Blood Proteins

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Freeman, W. M., Gooch, R. S., Lull, M. E., Worst, T. J., Walker, S. J., Xu, A. S. L., ... Vrana, K. E. (2006). Apo-AII is an elevated biomaker of chronic non-human primate ethanol self-administration. Alcohol and Alcoholism, 41(3), 300-305. https://doi.org/10.1093/alcalc/agl021

Apo-AII is an elevated biomaker of chronic non-human primate ethanol self-administration. / Freeman, Willard M.; Gooch, Randy S.; Lull, Melinda E.; Worst, Travis J.; Walker, Stephen J.; Xu, Arron S L; Green, Heather; Pierre, Peter J.; Grant, Kathleen (Kathy); Vrana, Kent E.

In: Alcohol and Alcoholism, Vol. 41, No. 3, 05.2006, p. 300-305.

Research output: Contribution to journalArticle

Freeman, WM, Gooch, RS, Lull, ME, Worst, TJ, Walker, SJ, Xu, ASL, Green, H, Pierre, PJ, Grant, KK & Vrana, KE 2006, 'Apo-AII is an elevated biomaker of chronic non-human primate ethanol self-administration', Alcohol and Alcoholism, vol. 41, no. 3, pp. 300-305. https://doi.org/10.1093/alcalc/agl021
Freeman, Willard M. ; Gooch, Randy S. ; Lull, Melinda E. ; Worst, Travis J. ; Walker, Stephen J. ; Xu, Arron S L ; Green, Heather ; Pierre, Peter J. ; Grant, Kathleen (Kathy) ; Vrana, Kent E. / Apo-AII is an elevated biomaker of chronic non-human primate ethanol self-administration. In: Alcohol and Alcoholism. 2006 ; Vol. 41, No. 3. pp. 300-305.
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AU - Walker, Stephen J.

AU - Xu, Arron S L

AU - Green, Heather

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