Apo-AII is an elevated biomaker of chronic non-human primate ethanol self-administration

Willard M. Freeman, Randy S. Gooch, Melinda E. Lull, Travis J. Worst, Stephen J. Walker, Arron S L Xu, Heather Green, Peter J. Pierre, Kathleen (Kathy) Grant, Kent E. Vrana

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16 Scopus citations

Abstract

Aims: Serum protein profiles were examined in näve, ethanol self-administering and ethanol abstinent cynomolgus monkeys (Macaca fasicularis) to search for differences in protein expression which could possibly serve as biomarkers of heavy ethanol consumption. Methods: Surface-enhanced laser desorption ionization time-of-flight (SELDI-ToF) mass spectrometry was used for proteomic profiling of serum. Results: Two proteins were identified by SELDI-ToF to be increased in ethanol self-administering compared with abstinent animals. These proteins were identified to be apolipoprotein AI (Apo-AI) and apolipoprotein AII (Apo-AII) by peptide mass fingerprinting and comparison with spectra of purified human Apo-AI and AII proteins. Immunoblot analysis of Apo-AI and Apo-AII was performed on a separate group of animals (within-animal ethanol-näve and self-administering) and confirmed a statistically significant increase in Apo-AII, while Apo-AI was unchanged. Conclusions: An open proteomic screen of serum and confirmation in a separate set of animals found Apo-AII to be increased in the serum of ethanol self-administering monkeys. These results are consistent with previous clinical studies of human ethanol consumption and serum apolipoprotein expression. Moreover, these results validate the use of non-human primates as a model organism for proteomic analysis of ethanol self-administration biomarkers.

Original languageEnglish (US)
Pages (from-to)300-305
Number of pages6
JournalAlcohol and Alcoholism
Volume41
Issue number3
DOIs
Publication statusPublished - May 2006
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Freeman, W. M., Gooch, R. S., Lull, M. E., Worst, T. J., Walker, S. J., Xu, A. S. L., ... Vrana, K. E. (2006). Apo-AII is an elevated biomaker of chronic non-human primate ethanol self-administration. Alcohol and Alcoholism, 41(3), 300-305. https://doi.org/10.1093/alcalc/agl021